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HISTOLOGIC ASSESSMENT OF TISSUE HEALING OF HYALINE CARTILAGE BY USE OF SEMIQUANTITATIVE EVALUATION SCALE

ANDREJA VUKASOVIĆ ; Department of Histology and Embryology, School of Medicine, University of Zagreb, Zagreb, Croatia
ALAN IVKOVIĆ ; Department of Orthopedics, Sveti Duh University Hospital, Zagreb, Sv. Katarina Special Hospital, Zabok, Croatia
DAVOR JEŽEK ; Department of Histology and Embryology, School of Medicine, University of Zagreb, Zagreb, Croatia
IVAN CEROVEČKI ; School of Medicine, University of Zagreb, Zagreb, Croatia
DRAŽEN VNUK ; Department of Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
MARIO KRESZINGER ; Department of Surgery, Orthopedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
DAMIR HUDETZ ; Department of Histology and Embryology, School of Medicine, University of Zagreb, Department of Orthopedics, Sveti Duh University Hospital, Zagreb, Sv. Katarina Special Hospital, Zabok, Croatia
MARKO PEĆINA ; School of Medicine, University of Zagreb, Zagreb, Croatia

Puni tekst: hrvatski, pdf (394 KB) str. 227-234 preuzimanja: 219* citiraj
APA 6th Edition
VUKASOVIĆ, A., IVKOVIĆ, A., JEŽEK, D., CEROVEČKI, I., VNUK, D., KRESZINGER, M., ... PEĆINA, M. (2011). HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE. Acta medica Croatica, 65 (3), 227-234. Preuzeto s https://hrcak.srce.hr/89154
MLA 8th Edition
VUKASOVIĆ, ANDREJA, et al. "HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE." Acta medica Croatica, vol. 65, br. 3, 2011, str. 227-234. https://hrcak.srce.hr/89154. Citirano 16.10.2019.
Chicago 17th Edition
VUKASOVIĆ, ANDREJA, ALAN IVKOVIĆ, DAVOR JEŽEK, IVAN CEROVEČKI, DRAŽEN VNUK, MARIO KRESZINGER, DAMIR HUDETZ i MARKO PEĆINA. "HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE." Acta medica Croatica 65, br. 3 (2011): 227-234. https://hrcak.srce.hr/89154
Harvard
VUKASOVIĆ, A., et al. (2011). 'HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE', Acta medica Croatica, 65(3), str. 227-234. Preuzeto s: https://hrcak.srce.hr/89154 (Datum pristupa: 16.10.2019.)
Vancouver
VUKASOVIĆ A, IVKOVIĆ A, JEŽEK D, CEROVEČKI I, VNUK D, KRESZINGER M i sur. HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE. Acta medica Croatica [Internet]. 2011 [pristupljeno 16.10.2019.];65(3):227-234. Dostupno na: https://hrcak.srce.hr/89154
IEEE
A. VUKASOVIĆ, et al., "HISTOLOŠKA PROCJENA CIJELJENJA ZGLOBNE HRSKAVICE UPOTREBOM SEMIKVANTITATIVNE OCJENSKE LJESTVICE", Acta medica Croatica, vol.65, br. 3, str. 227-234, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/89154. [Citirano: 16.10.2019.]

Sažetak
Introduction: Articular cartilage is an avascular and aneural tissue lacking lymph drainage, hence its inability of spontaneous repair following injury. Thus, it offers an interesting model for scientific research. A number of methods have been suggested to enhance cartilage repair, but none has yet produced significant success. The possible application of the aforementioned methods has brought about the necessity to evaluate their results. The objective of this study was to analyze results of a study of the effects of the use of TGF-β gene transduced bone marrow clot on articular cartilage defects using ICRS visual histological assessment scale. Methods: The research was conducted on 28 skeletally mature sheep that were randomly assigned to four groups and surgically inflicted femoral chondral defects. The articular surfaces were then treated with TGF-β1 gene transduced bone marrow clot (TGF group), GFP transduced bone marrow clot (GFP group), untransduced bone marrow clot (BM group) or left untreated (NC group). The analysis was performed by visual examination of cartilage samples and results were obtained using ICRS visual histological assessment scale. The results were subsequently subjected to statistical assessment using Kruskal-Wallis and Mann-Whitney tests. Results: Kruskal-Wallis test yielded statistically significant difference with respect to cell distribution. Mann-Whitney test showed statistically significant difference between TGF and NC groups (P=0.002), as well as between BM and NC groups (P=0.002 with Bonferroni correction).
Discussion: Twenty-six of the twenty-eight samples were subjected to histologic and subsequent statistical analysis; two were discarded due to faulty histology technique. Our results indicated a level of certainty as to the positive effect of TGF-β1 gene transduced bone marrow clot in restoration of articular cartilage defects. However, additional research is necessary in the field. One of the significant drawbacks on histologic assessment of cartilage samples were the errors in histologic preparation, for which some samples had to be discarded and significantly impaired the analytical quality of the others. Defects of structures surrounding the articular cartilage, e.g., subchondral bone or connective tissue, might also impair the quality of histologic analysis. Additional analyses, i.e. polarizing microscopy should be performed to determine the degree of integration of the newly formed tissue with the surrounding cartilage. The semiquantitative ICRS scale, although of great practical value, has limitations as to the objectivity of the assessment, taking into account the analytical ability of the evaluator, as well as the accuracy of emiquantitative analysis in comparison to the methods of quantitative analysis.Conclusion: Overall results of histologic analysis indicated that the application of TGF-β1 gene transduced bone marrow clot could have measurable clinical effects on articular cartilage repair. The ICRS visual histological assessment scale is a valuable analytical method for cartilage repair evaluation. In this respect, further analyses of the method value would be of great importance.

Ključne riječi
hyaline cartilage; cartilage repair; ICRS visual histological assessment scale

Hrčak ID: 89154

URI
https://hrcak.srce.hr/89154

[hrvatski]

Posjeta: 599 *