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Arhiv za higijenu rada i toksikologiju, Vol. 51 No. 1, 2000.

Izvorni znanstveni članak

Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method

Elsa Reiner
Goran Šinko
Mira Škrinjarić-Špoljar
Vera Simeon-Rudolf

Puni tekst: engleski, pdf (324 KB) str. 13-18 preuzimanja: 3.714* citiraj
APA 6th Edition
Reiner, E., Šinko, G., Škrinjarić-Špoljar, M. i Simeon-Rudolf, V. (2000). Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method. Arhiv za higijenu rada i toksikologiju, 51 (1), 13-18. Preuzeto s https://hrcak.srce.hr/884
MLA 8th Edition
Reiner, Elsa, et al. "Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method." Arhiv za higijenu rada i toksikologiju, vol. 51, br. 1, 2000, str. 13-18. https://hrcak.srce.hr/884. Citirano 18.02.2019.
Chicago 17th Edition
Reiner, Elsa, Goran Šinko, Mira Škrinjarić-Špoljar i Vera Simeon-Rudolf. "Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method." Arhiv za higijenu rada i toksikologiju 51, br. 1 (2000): 13-18. https://hrcak.srce.hr/884
Harvard
Reiner, E., et al. (2000). 'Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method', Arhiv za higijenu rada i toksikologiju, 51(1), str. 13-18. Preuzeto s: https://hrcak.srce.hr/884 (Datum pristupa: 18.02.2019.)
Vancouver
Reiner E, Šinko G, Škrinjarić-Špoljar M, Simeon-Rudolf V. Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method. Arh Hig Rada Toksikol. [Internet]. 2000 [pristupljeno 18.02.2019.];51(1):13-18. Dostupno na: https://hrcak.srce.hr/884
IEEE
E. Reiner, G. Šinko, M. Škrinjarić-Špoljar i V. Simeon-Rudolf, "Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method", Arhiv za higijenu rada i toksikologiju, vol.51, br. 1, str. 13-18, 2000. [Online]. Dostupno na: https://hrcak.srce.hr/884. [Citirano: 18.02.2019.]

Sažetak
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the advantages and disadvantages of each and concludes with a proposal for an international agreement between laboratories for the evaluation of a standardized protocol.

Ključne riječi
acetylcholinesterase; butyrylcholinesterase; human erythrocytes; human plasma

Hrčak ID: 884

URI
https://hrcak.srce.hr/884

[hrvatski]

Posjeta: 4.471 *