Biochemia Medica, Vol. 16 No. 1, 2006.
Izvorni znanstveni članak
Concentration of malondialdehyde in a NOD mice treated with acarbose
Roberta Petlevski
Dubravka Juretić
Mirko Hadžija
Milivoj Slijepčević
Jana Lukač-Bajalo
Sažetak
Aim. Malondialdehyde (MDA) is one of the toxic product of the lipid peroxida-tion, and that plays an important role in the evaluation of oxidative stress in the diabetes mellitus. The aim of this study was to study the effect of acarbo-se (α-glucosidase inhibitor) on serum glucose concentration and MDA in liver homogenate of NOD (non-obese diabetic) mice.
Material and methods. Diabetes was induced in mice by i.v. administration of alloxan-monohydrate (75 mg/kg b.w.). The mice were divided into 4 groups (N=6): control, healthy mice (C), control healthy mice on 7-day treatment with acarbose (C/A), NOD mice (D), and NOD mice on 7-day acarbose treatment (D/A).
The levels of blood glucose and of MDA in liver homogenate were determined by glucose oxidase-peroxidase method and a method with thiobarbituric acid, respectively.
Results. Significant reduction in blood glucose levels was observed after 7-day administration of acarbose in D/A group compared to D group (p<0.05), together with a significant fall in MDA concentration (p<0.05).
Conclusion. This results confirmed thefavorable antioxidative effect of acarbose which may be explained by its antihyperglycemic action.
Ključne riječi
oxidative stress; diabetes; lipid peroxidation; acarbose
Hrčak ID:
11534
URI
Datum izdavanja:
15.5.2006.
Posjeta: 1.825 *