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HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products

A. Krivohlavek
L. Barušić
Z. Šmit
J. Bošnir
D. Puntarić

Puni tekst: engleski, pdf (558 KB) str. 53-56 preuzimanja: 633* citiraj
APA 6th Edition
Krivohlavek, A., Barušić, L., Šmit, Z., Bošnir, J. i Puntarić, D. (2007). HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products. Kemija u industriji, 56 (2), 53-56. Preuzeto s https://hrcak.srce.hr/12338
MLA 8th Edition
Krivohlavek, A., et al. "HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products." Kemija u industriji, vol. 56, br. 2, 2007, str. 53-56. https://hrcak.srce.hr/12338. Citirano 24.10.2019.
Chicago 17th Edition
Krivohlavek, A., L. Barušić, Z. Šmit, J. Bošnir i D. Puntarić. "HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products." Kemija u industriji 56, br. 2 (2007): 53-56. https://hrcak.srce.hr/12338
Harvard
Krivohlavek, A., et al. (2007). 'HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products', Kemija u industriji, 56(2), str. 53-56. Preuzeto s: https://hrcak.srce.hr/12338 (Datum pristupa: 24.10.2019.)
Vancouver
Krivohlavek A, Barušić L, Šmit Z, Bošnir J, Puntarić D. HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products. Kemija u industriji [Internet]. 2007 [pristupljeno 24.10.2019.];56(2):53-56. Dostupno na: https://hrcak.srce.hr/12338
IEEE
A. Krivohlavek, L. Barušić, Z. Šmit, J. Bošnir i D. Puntarić, "HPLC-MS Analysis of Chloramphenicol Residues in Milk and Powdered Milk Products", Kemija u industriji, vol.56, br. 2, str. 53-56, 2007. [Online]. Dostupno na: https://hrcak.srce.hr/12338. [Citirano: 24.10.2019.]

Sažetak
Chloramphenicol (CAP) is a broad-spectrum antibiotic with bacteriostatic action but also has toxic properties, which is why its presence in food and feed is prohibited in Croatia and the European Union.
In the aim of consumer protection it is essential to develop a sensitive analytical method for detection of CAP fractions lower than w = 0.3 µg kg--1-. For the efficient control and monitoring of CAP, a rapid, sensitive, and selective method for its identification and quantification, using high
performance liquid chromatography in combination with mass spectrometry LC-MS, has been developed.
The cleaning procedure was based on the AOAC official method 993.32. HPLC-MS analysis used the ODS Hypersile column and the water/acetonitrile gradient. Electrospray negative ionization (neg ESI) was used before single ion monitoring (SIM) detection of three m/z 321, 323 and 325. As additional criteria, the ratio between these masses in real and spiked milk samples was also investigated in accordance with theoretical values of the isotope pattern for 2 chlorine atoms present in the analyte.
The detection limit of 0.1 µg kg--1- was achieved. The mean value of recovery was 94 %, the correlation coefficient of the calibration curves calculated for 2 m/z values was higher than 0.99.
Fourty samples of milk and milk products were tested with the HPLC-MS method, and obtained results showed that samples had CAP 0.37, 0.29, 0.39 µg kg--1-, respectively. All the other analysed samples contained CAP concentrations below the detection limit.

Ključne riječi
chloramphenicol; liquid chromatography; mass spectrometry; milk

Hrčak ID: 12338

URI
https://hrcak.srce.hr/12338

[hrvatski]

Posjeta: 1.275 *