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Original scientific paper
https://doi.org/10.15567/mljekarstvo.2015.0104

Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)

Hayriye Yesim Can   ORCID icon orcid.org/0000-0002-5191-6268 ; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey
Mehmet Elmalı ; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31034 Hatay, Turkey
Alper Karagöz ; Microbiology Reference Laboratories Department, Public Health Institutes of Turkey, 06100 Ankara, Turkey

Fulltext: english, pdf (540 KB) pages 26-31 downloads: 777* cite
APA 6th Edition
Can, H.Y., Elmalı, M. & Karagöz, A. (2015). Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR). Mljekarstvo, 65 (1), 26-31. https://doi.org/10.15567/mljekarstvo.2015.0104
MLA 8th Edition
Can, Hayriye Yesim, et al. "Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)." Mljekarstvo, vol. 65, no. 1, 2015, pp. 26-31. https://doi.org/10.15567/mljekarstvo.2015.0104. Accessed 8 Dec. 2019.
Chicago 17th Edition
Can, Hayriye Yesim, Mehmet Elmalı and Alper Karagöz. "Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)." Mljekarstvo 65, no. 1 (2015): 26-31. https://doi.org/10.15567/mljekarstvo.2015.0104
Harvard
Can, H.Y., Elmalı, M., and Karagöz, A. (2015). 'Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)', Mljekarstvo, 65(1), pp. 26-31. https://doi.org/10.15567/mljekarstvo.2015.0104
Vancouver
Can HY, Elmalı M, Karagöz A. Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR). Mljekarstvo [Internet]. 2015 [cited 2019 December 08];65(1):26-31. https://doi.org/10.15567/mljekarstvo.2015.0104
IEEE
H.Y. Can, M. Elmalı and A. Karagöz, "Detection of Coxiella burnetii in cows’, goats’, and ewes’ bulk milk samples using polymerase chain reaction (PCR)", Mljekarstvo, vol.65, no. 1, pp. 26-31, 2015. [Online]. https://doi.org/10.15567/mljekarstvo.2015.0104

Abstracts
Q fever is a widespread zoonotic disease that is caused by obligate intracellular bacteria, Coxiella burnetii. This study was planned to determine the prevalence of C. burnetii in cows’, goats’, and ewes’ bulk milk (BM) samples using PCR and to confirm positive results by DNA sequencing. A total of 150 BM samples (50 samples of each cows’, goats’, and ewes’ milk) collected from 15 randomly selected dairy farms in Hatay province were analyzed. The BM samples were taken between January 2012 and July 2013. Bacterial DNA was extracted directly from milk samples. Nine of the BM samples (6 %) were PCR positive; five from cows’ BM, two from ewes’ BM, and two from goats’ BM. Bacterial DNA was detected in 3 of 15 (20 %) dairy farms. Positive results were confirmed by DNA sequencing. The sequencing results of Coxiella DNA extracted from cows’, goats’, and ewes’ milk samples were consistent with the reference strain with 100 %, 99.7 %, 99.8 % homology, respectively. BM samples were found to be contaminated with C. burnetii; therefore, taking hygienic measures is necessary for food safety and public health.

Keywords
bulk milk; Coxiella; polymerase chain reaction; sequence analysis

Hrčak ID: 133532

URI
https://hrcak.srce.hr/133532

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