Acta Botanica Croatica, Vol. 29 No. 1, 1970.
Original scientific paper
Occurrence of 5-hydroxytryptamine in the Roman Nettle (Urtica pilulifera L.)
Ivan Regula
; Hrvatska
Abstract
Leaves and stems of the Roman nettle (Urtica pilulifera) were frozen, macerated in a blendor in 80% methanol at 5°C, and the plant material repeatedly extracted with methanol for 22 hrs at +2 °C. The separation of the basic compounds was achieved on a Amberlite CG-50 column. The identification of 5-hydroxytryptamine was based on Rf-values (compared to those of an authentic sample of 5-hydroxytryptamine), colour reactions with several reagents and fluorescence characteristics. The amount found was 0.4 p,g/g of fresh weight. Histochemical investigations with p-dimethylamino-cinnamaldehyde indicate that 5-hydroxytryptamine is located mainly in the stings.
Keywords
Hrčak ID:
156341
URI
Publication date:
31.12.1970.
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