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https://doi.org/10.5599/admet.4.2.314

Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells

Gregory B. Pott ; Eastern Colorado Health Care System, Denver Colorado
Mark Tsurudome ; Eastern Colorado Health Care System, Denver Colorado
Jamie Bui ; Eastern Colorado Health Care System, Denver Colorado
Chelsea Gardner ; Eastern Colorado Health Care System, Denver Colorado
Marc Goalstone ; Eastern Colorado Health Care System, Denver Colorado

Puni tekst: engleski, pdf (774 KB) str. 232-240 preuzimanja: 301* citiraj
APA 6th Edition
Pott, G.B., Tsurudome, M., Bui, J., Gardner, C. i Goalstone, M. (2016). Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells. ADMET and DMPK, 4 (3), 232-240. https://doi.org/10.5599/admet.4.2.314
MLA 8th Edition
Pott, Gregory B., et al. "Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells." ADMET and DMPK, vol. 4, br. 3, 2016, str. 232-240. https://doi.org/10.5599/admet.4.2.314. Citirano 19.10.2021.
Chicago 17th Edition
Pott, Gregory B., Mark Tsurudome, Jamie Bui, Chelsea Gardner i Marc Goalstone. "Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells." ADMET and DMPK 4, br. 3 (2016): 232-240. https://doi.org/10.5599/admet.4.2.314
Harvard
Pott, G.B., et al. (2016). 'Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells', ADMET and DMPK, 4(3), str. 232-240. https://doi.org/10.5599/admet.4.2.314
Vancouver
Pott GB, Tsurudome M, Bui J, Gardner C, Goalstone M. Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells. ADMET and DMPK [Internet]. 2016 [pristupljeno 19.10.2021.];4(3):232-240. https://doi.org/10.5599/admet.4.2.314
IEEE
G.B. Pott, M. Tsurudome, J. Bui, C. Gardner i M. Goalstone, "Cross talk of c-Jun N-terminal kinase and p38 map kinase modulate insulin and TNFα-stimulated VCAM-1 expression in rat aorta endothelial cells", ADMET and DMPK, vol.4, br. 3, str. 232-240, 2016. [Online]. https://doi.org/10.5599/admet.4.2.314

Sažetak
VCAM-1 cell surface expression was determined by flow cytometry in rat aorta endothelial cells stimulated with insulin and/or Tumor Necrosis Factor-a in the absence or presence of short-hairpin RNA inhibitors of c-Jun N-terminal Kinase (JNK) and p38 MAP Kinase. Cells transfected with insulin alone exhibited moderate increases in cell surface VCAM-1 expression, whereas cells stimulated with TNFα alone or in combination with insulin, exhibited a significant (P, 0.05) increase in VCAM-1 surface expression. Cells transfected with shJNK alone showed increased VCAM-1 expression at the cell surface as compared to mock-transfected positive controls. In contrast, cells transfected with shp38 exhibited significant decreased insulin-stimulated VCAM-1 expression at the cell surface, but only moderate decreased VCAM-1 expression in cells stimulated by TNFα alone in combination with insulin. Interestingly, in cells first tranfected with shJNK and then shp38, VCAM-1 expression appeared to increase in an additive fashion. In contrast, cells transfected with shp38 first then shJNK, VCAM-1 expression exhibited increased VCAM-1 expression even in the presence of shp38. One can conclude that JNK is a potent negative regulator of insulin- and TNFα-stimulated cell surface expression of VCAM-1, whereas p38 is mild positive regulator of insulin-, but not TNFα-stimulated VCAM-1 expression. JNK appears to be a more potent mediator of insulin- and TNFα-stimulated cells surface VCAM-1 expression than p38 MAP Kinase. Thus, it could be a therapeutic target for amelioration of inflammation-associated VCAM-1 expression and its sequelae of events that lead to atherosclerosis.

Ključne riječi
JNK; p38, TNFα; VCAM-1; Hyperinsulinemia; Atherosclerosis

Hrčak ID: 167044

URI
https://hrcak.srce.hr/167044

Posjeta: 525 *