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https://doi.org/10.3325/cmj.2016.57.51

Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model

Lei Hao ; Department of Neurology, No 324 Hospital of People’s LiberationArmy, Chongqing, China
Xiuming Guo ; Department of Neurology The First Affiliated Hospital of Chongqing Medical University,Chongqing, China
Can Zou ; Department of Neurology, No 324 Hospital of People’s LiberationArmy, Chongqing, China
Huchuan Zhou ; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Hong Tian ; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Yubo Zhang ; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Chuan Song ; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China
Lei Liu ; Department of Neurology, No 324 Hospital of People’s Liberation Army, Chongqing, China

Puni tekst: engleski, pdf (2 MB) str. 51-57 preuzimanja: 336* citiraj
APA 6th Edition
Hao, L., Guo, X., Zou, C., Zhou, H., Tian, H., Zhang, Y., ... Liu, L. (2016). Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model. Croatian Medical Journal, 57 (1), 51-57. https://doi.org/10.3325/cmj.2016.57.51
MLA 8th Edition
Hao, Lei, et al. "Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model." Croatian Medical Journal, vol. 57, br. 1, 2016, str. 51-57. https://doi.org/10.3325/cmj.2016.57.51. Citirano 28.10.2021.
Chicago 17th Edition
Hao, Lei, Xiuming Guo, Can Zou, Huchuan Zhou, Hong Tian, Yubo Zhang, Chuan Song i Lei Liu. "Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model." Croatian Medical Journal 57, br. 1 (2016): 51-57. https://doi.org/10.3325/cmj.2016.57.51
Harvard
Hao, L., et al. (2016). 'Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model', Croatian Medical Journal, 57(1), str. 51-57. https://doi.org/10.3325/cmj.2016.57.51
Vancouver
Hao L, Guo X, Zou C, Zhou H, Tian H, Zhang Y i sur. Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model. Croat Med J. [Internet]. 2016 [pristupljeno 28.10.2021.];57(1):51-57. https://doi.org/10.3325/cmj.2016.57.51
IEEE
L. Hao, et al., "Hyperbaric oxygen preconditioning ameliorates blood-brain barrier damage induced by hypoxia through modulation of tight junction proteins in an in vitro model", Croatian Medical Journal, vol.57, br. 1, str. 51-57, 2016. [Online]. https://doi.org/10.3325/cmj.2016.57.51

Sažetak
Aim To explore the effects of hyperbaric oxygen preconditioning
(HBOP) on the permeability of blood-brain barrier
(BBB) and expression of tight junction proteins under hypoxic
conditions in vitro.
Methods A BBB in vitro model was constructed using the
hCMEC/D3 cell line and used when its trans-endothelial
electrical resistance (TEER) reached 80-120 Ω · cm2 (tested
by Millicell-Electrical Resistance System). The cells were
randomly divided into the control group cultured under
normal conditions, the group cultured under hypoxic conditions
(2%O2) for 24 h (hypoxia group), and the group
first subjected to HBOP for 2 h and then to hypoxia (HBOP
group). Occludin and ZO-1 expression were analyzed by
immunofluorescence assay.
Results Normal hCMEC/D3 was spindle-shaped and
tightly integrated. TEER was significantly reduced in the
hypoxia (P = 0.001) and HBOP group (P = 0.014) compared
to control group, with a greater decrease in the hypoxia
group. Occludin membranous expression was significantly
decreased in the hypoxia group (P = 0.001) compared to
the control group, but there was no change in the HBOP
group. ZO-1 membranous expression was significantly decreased
(P = 0.002) and cytoplasmic expression was significantly
increased (P = 0.001) in the hypoxia group compared
to the control group, although overall expression
levels did not change. In the HBOP group, there was no
significant change in ZO-1 expression compared to the
control group.
Conclusion Hyperbaric oxygen preconditioning protected
the integrity of BBB in an in vitro model through modulation
of occludin and ZO-1 expression under hypoxic conditions.

Hrčak ID: 169153

URI
https://hrcak.srce.hr/169153

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