Prethodno priopćenje
https://doi.org/10.24141/1/3/2/9
Development of Immunohistochemical Staining Methods and Optimization of Immunohistochemical Protocol for BACH2 Transcription Factor
Nikolina Vukoja
; Odjel za kliničko-laboratorijsku dijagnostiku, Dječja bolnica Srebrnjak, Zagreb, Hrvatska
Suzana Hančić
; Klinički zavod za patologiju i citologiju, KB Merkur, Zagreb, Hrvatska
Petra Korać
orcid.org/0000-0003-4783-2114
; Zavod za molekularnu biologiju, Biološki odsjek, Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sažetak
Immunohistochemical staining is a method for detection of presence and localization of specific antigens in cells and/or tissues. It is based on the basic principle of immunoreactions: binding of specific antibody to a certain antigen. Development of immunohistochemical staining methods started during the 1930s and, in a number of variations, they are still used today both in research and routine diagnostic procedures.
This paper offers presentation of immunohistochemical methods development and gives an example of protocol optimization for detection of BACH2 antigen in human secondary lymphoid tissue. Most important steps of the protocol are discussed (section preparation, antigen demasking and specific antibody incubation conditions). Optimal protocol for immunohistochemical staining for BACH2 antigen is given and can be used in any routine hematopathological laboratory.
Ključne riječi
immunohistochemical staining; BACH2; epitope demasking; antibody incubation
Hrčak ID:
190986
URI
Datum izdavanja:
18.12.2017.
Posjeta: 3.352 *