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Acid Proteinases from Calf Lymph Nodes

Tamara Turnšek ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
I. Kregar ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
D. Lebez ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia

Puni tekst: engleski, pdf (11 MB) str. 59-69 preuzimanja: 57* citiraj
APA 6th Edition
Turnšek, T., Kregar, I. i Lebez, D. (1975). Acid Proteinases from Calf Lymph Nodes. Croatica Chemica Acta, 47 (1), 59-69. Preuzeto s https://hrcak.srce.hr/196695
MLA 8th Edition
Turnšek, Tamara, et al. "Acid Proteinases from Calf Lymph Nodes." Croatica Chemica Acta, vol. 47, br. 1, 1975, str. 59-69. https://hrcak.srce.hr/196695. Citirano 09.03.2021.
Chicago 17th Edition
Turnšek, Tamara, I. Kregar i D. Lebez. "Acid Proteinases from Calf Lymph Nodes." Croatica Chemica Acta 47, br. 1 (1975): 59-69. https://hrcak.srce.hr/196695
Harvard
Turnšek, T., Kregar, I., i Lebez, D. (1975). 'Acid Proteinases from Calf Lymph Nodes', Croatica Chemica Acta, 47(1), str. 59-69. Preuzeto s: https://hrcak.srce.hr/196695 (Datum pristupa: 09.03.2021.)
Vancouver
Turnšek T, Kregar I, Lebez D. Acid Proteinases from Calf Lymph Nodes. Croatica Chemica Acta [Internet]. 1975 [pristupljeno 09.03.2021.];47(1):59-69. Dostupno na: https://hrcak.srce.hr/196695
IEEE
T. Turnšek, I. Kregar i D. Lebez, "Acid Proteinases from Calf Lymph Nodes", Croatica Chemica Acta, vol.47, br. 1, str. 59-69, 1975. [Online]. Dostupno na: https://hrcak.srce.hr/196695. [Citirano: 09.03.2021.]

Sažetak
Acid proteinases were isolated from calf lymph nodes using
acid extraction, ammonium sulphate and acetone precipitation,
followed by ion exchange chromatography on CM-cellulose and
_ gel chromatography on Sephadex G-100. Cathepsin D (E. C. 3.4.23.5)
is present in lymph nodes. It has the molecular weight of 39 000
as determined by gel filtration on Sephadex G-100. Cathepsins
Bl and B2 (E. C. 3.4.22.1) were also isolated. Molecular weights
of 22 000 and 51 000 were determined for cathepsins Bl and B2,
respectively. Calf lymph nodes contain also another proteinase
which degrades haemoglobin at an optimum at pH = 3.0. This
proteinase has a molecular weight of 14 000 as was determined
by gel filtration. Its activity is not inhibited with 0,25 ~tM pepstatin
which inhibits 903/o of the cathepsin D activity.

Hrčak ID: 196695

URI
https://hrcak.srce.hr/196695

Posjeta: 106 *