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Pregledni rad
https://doi.org/10.5599/admet.535

Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices

Cayman Stephen ; Department of Biological Sciences, The University of Alabama, Science and Engineering Complex 2329 Tuscaloosa, AL 35487, USA
Abdelfatteh El Omri ; Genomics and Biotechnology Section and Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah 21589, Box 80141, Saudi Arabia
Lukasz Ciesla ; Department of Biological Sciences, The University of Alabama, Science and Engineering Complex 2329 Tuscaloosa, AL 35487, USA

Puni tekst: engleski, pdf (1 MB) str. 200-214 preuzimanja: 183* citiraj
APA 6th Edition
Stephen, C., El Omri, A. i Ciesla, L. (2018). Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices. ADMET and DMPK, 6 (3), 200-214. https://doi.org/10.5599/admet.535
MLA 8th Edition
Stephen, Cayman, et al. "Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices." ADMET and DMPK, vol. 6, br. 3, 2018, str. 200-214. https://doi.org/10.5599/admet.535. Citirano 22.11.2019.
Chicago 17th Edition
Stephen, Cayman, Abdelfatteh El Omri i Lukasz Ciesla. "Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices." ADMET and DMPK 6, br. 3 (2018): 200-214. https://doi.org/10.5599/admet.535
Harvard
Stephen, C., El Omri, A., i Ciesla, L. (2018). 'Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices', ADMET and DMPK, 6(3), str. 200-214. https://doi.org/10.5599/admet.535
Vancouver
Stephen C, El Omri A, Ciesla L. Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices. ADMET and DMPK [Internet]. 2018 [pristupljeno 22.11.2019.];6(3):200-214. https://doi.org/10.5599/admet.535
IEEE
C. Stephen, A. El Omri i L. Ciesla, "Cellular membrane affinity chromatography (CMAC) in drug discovery from complex natural matrices", ADMET and DMPK, vol.6, br. 3, str. 200-214, 2018. [Online]. https://doi.org/10.5599/admet.535

Sažetak
Secondary plant metabolites are evolutionary-designed molecules that interact with multiple biological targets in human organisms. Identification of pharmacologically active phytochemicals is usually a time consuming and costly process. Cellular membrane affinity chromatography (CMAC) allows the detection of secondary metabolites present in complex natural matrices, e.g. plant extracts and their interactions with the immobilized fully-functional transmembrane proteins. After the isolation process of the binding compounds, CMAC columns can be used to study the binding process between the potential new ligands and the immobilized transmembrane protein target. The following parameters can be determined using CMAC columns: binding affinity (Kd), association rate constant (kon), dissociation rate constant (koff) and the equilibrium constant for complex formation (K). This review summarizes the preparation steps and the use of CMAC columns in the drug discovery process of new potential drug leads present in complex natural matrices.

Ključne riječi
artificial membrane stationary phase; bioassay; chromatography; frontal affinity chromatography; IAM.PC; immobilized protein; phytochemicals; plant extracts

Hrčak ID: 205902

URI
https://hrcak.srce.hr/205902

Posjeta: 269 *