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Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs

Blagica Jovanova   ORCID icon orcid.org/ORCID ; Department of Toxicology, Institute of Applied Biochemistry, Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, Republic of Macedonia
Svetlana Kulevanova   ORCID icon orcid.org/ORCID ; Department of Pharmacognosy, Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, Republic of Macedonia
Tatjana Kadifkova Panovska   ORCID icon orcid.org/ORCID ; Department of Toxicology, Institute of Applied Biochemistry, Faculty of Pharmacy, Ss. Cyril and Methodius University, Skopje, Republic of Macedonia

Puni tekst: engleski, pdf (622 KB) str. 51-58 preuzimanja: 370* citiraj
APA 6th Edition
Jovanova, B., Kulevanova, S. i Kadifkova Panovska, T. (2019). Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs. Agriculturae Conspectus Scientificus, 84 (1), 51-58. Preuzeto s https://hrcak.srce.hr/218535
MLA 8th Edition
Jovanova, Blagica, et al. "Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs." Agriculturae Conspectus Scientificus, vol. 84, br. 1, 2019, str. 51-58. https://hrcak.srce.hr/218535. Citirano 05.12.2020.
Chicago 17th Edition
Jovanova, Blagica, Svetlana Kulevanova i Tatjana Kadifkova Panovska. "Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs." Agriculturae Conspectus Scientificus 84, br. 1 (2019): 51-58. https://hrcak.srce.hr/218535
Harvard
Jovanova, B., Kulevanova, S., i Kadifkova Panovska, T. (2019). 'Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs', Agriculturae Conspectus Scientificus, 84(1), str. 51-58. Preuzeto s: https://hrcak.srce.hr/218535 (Datum pristupa: 05.12.2020.)
Vancouver
Jovanova B, Kulevanova S, Kadifkova Panovska T. Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs. Agriculturae Conspectus Scientificus [Internet]. 2019 [pristupljeno 05.12.2020.];84(1):51-58. Dostupno na: https://hrcak.srce.hr/218535
IEEE
B. Jovanova, S. Kulevanova i T. Kadifkova Panovska, "Determination of the Total Phenolic Content, Antioxidant Activity and Cytotoxicity of Selected Aromatic Herbs", Agriculturae Conspectus Scientificus, vol.84, br. 1, str. 51-58, 2019. [Online]. Dostupno na: https://hrcak.srce.hr/218535. [Citirano: 05.12.2020.]

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Sažetak
Aromatic plants used as culinary herbs contain phytochemicals with distinct properties affecting the population that utilizes them, yet there is still insufficient data on their bioactive profile. The present study investigated the antioxidant and cytotoxic properties of five aromatic herbs: Allium schoenoprasum L. (ASPR), Allium ursinum L. (AUR), Anthriscus cerefolium L. Hoffm. (ACH), Capsicum annuum L. var. annuum (CAF) and Foeniculum vulgare Mill (FVH). Total phenolic content (TPC) and total flavonoid content (TFC) were determined by Folin- Ciocalteu method and AlCl3 method. Antioxidant activity of the extracts was examined by 2,2’-diphenyl-1-pycrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), Non- site-specific-degradation (NSSOH) and Site-specific-deoxyribose-degradation (SSOH) assays. The cytotoxicity of the extracts was evaluated by Brine shrimp lethality assay (BSLA). Considerable variations were observed for TPC values from 65.03 to 253.74 mg GAE/g crude extract and TFC values from 8.02 to 49.58 mg QE/g crude extract. Highest quantity of total polyphenols and flavonoids was measured in CAF, which also demonstrated strong radical scavenging ability, reducing power and chelating activity. ACH showed lower amount of polyphenols and weak antioxidant activity. Obtained LC50 values by BSLA revealed strong cytotoxicity for CAF, moderate for FVH and ASPR, weak cytotoxicity for AUR, while ACH caused no toxic effects against the shrimps.

Ključne riječi
dietary antioxidants; antioxidant capacity; cytotoxic potential; brine shrimp lethality assay; LC50

Hrčak ID: 218535

URI
https://hrcak.srce.hr/218535

Posjeta: 532 *