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Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population

Mohammed Baqur Al-Shuhaib   ORCID icon orcid.org/ORCID ; Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq
Tahreer M. Al-Thuwaini   ORCID icon orcid.org/ORCID ; Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq
Rawan A. Al-Fihan   ORCID icon orcid.org/ORCID ; Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, Iraq
Ali A. Al-Qutbi   ORCID icon orcid.org/ORCID ; Department of Food Science and Technology, College of Food Science, Al-Qasim Green University, Al-Qasim, Babil, Iraq

Puni tekst: engleski, pdf (749 KB) str. 211-218 preuzimanja: 152* citiraj
APA 6th Edition
Al-Shuhaib, M.B., Al-Thuwaini, T.M., Al-Fihan, R.A. i Al-Qutbi, A.A. (2019). Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population. Agriculturae Conspectus Scientificus, 84 (2), 211-218. Preuzeto s https://hrcak.srce.hr/221766
MLA 8th Edition
Al-Shuhaib, Mohammed Baqur, et al. "Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population." Agriculturae Conspectus Scientificus, vol. 84, br. 2, 2019, str. 211-218. https://hrcak.srce.hr/221766. Citirano 28.10.2021.
Chicago 17th Edition
Al-Shuhaib, Mohammed Baqur, Tahreer M. Al-Thuwaini, Rawan A. Al-Fihan i Ali A. Al-Qutbi. "Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population." Agriculturae Conspectus Scientificus 84, br. 2 (2019): 211-218. https://hrcak.srce.hr/221766
Harvard
Al-Shuhaib, M.B., et al. (2019). 'Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population', Agriculturae Conspectus Scientificus, 84(2), str. 211-218. Preuzeto s: https://hrcak.srce.hr/221766 (Datum pristupa: 28.10.2021.)
Vancouver
Al-Shuhaib MB, Al-Thuwaini TM, Al-Fihan RA, Al-Qutbi AA. Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population. Agriculturae Conspectus Scientificus [Internet]. 2019 [pristupljeno 28.10.2021.];84(2):211-218. Dostupno na: https://hrcak.srce.hr/221766
IEEE
M.B. Al-Shuhaib, T.M. Al-Thuwaini, R.A. Al-Fihan i A.A. Al-Qutbi, "Genotyping of Diacylglycerol Acyltransferase 2 Gene in Holstein Cattle Population", Agriculturae Conspectus Scientificus, vol.84, br. 2, str. 211-218, 2019. [Online]. Dostupno na: https://hrcak.srce.hr/221766. [Citirano: 28.10.2021.]

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Sažetak
This study was conducted to describe the single nucleotide polymorphism (SNPs) within exon 8 and its intron 9 flanking region within bovine Diacylglycerol Acyltransferase 2 (DGAT2) gene and the possible association of these SNPs with the milk productive traits. Blood samples were collected from randomly selected Holstein cattle. Genomic DNA was extracted, and a pair of specific PCR primers was designed to amplify a segment that consists of partial exon 8/partial intron 9 of DGAT2 gene. SSCP experiments were optimized and performed for each amplified PCR fragment. Each set of SSCP resolved bands was sequenced and analyzed. Four SSCP patterns representing four genotypes (BD, BB, CD, and AD) were detected with four alleles. Several novel genetic polymorphisms were discovered. Three SNPs (157 C/A, 158 T/G, and 159 G/A) were found in genotype BD,  while one SNP (94 G/T,  153 C/A, 154 T/A) were found in the genotypes BB, CD, and AD, respectively. The only non-synonymous SNP was found in genotype CD (344 D/Y), and the effect of this missense mutation on the protein three-dimensional structure was visualized. The sequence homology between the bovine DGAT2 gene and other species was also analyzed. The significance of the correlation of each genotype with the productive traits of milk was observed. The Holstein cattle with BD genotype produced significantly more milk for all studied 90 days of lactation, with a significant effect on fat for only the last 30 days of lactation, whereas there was no effect on protein and lactose percentage for the entire lactation period. The results suggest a novel association between bovine DGAT2 genetic variability and the milk yield in Holstein cattle. This opens interesting prospects for future DGAT2 based selection programs and preservation strategies.

Ključne riječi
Holstein cattle; DGAT2 gene; genotyping; protein structure; milk yield

Hrčak ID: 221766

URI
https://hrcak.srce.hr/221766

Posjeta: 308 *