Izvorni znanstveni članak
https://doi.org/10.17113/ftb.57.02.19.6142
Influence of Dehydrated Wheat/Rice Cereal Matrices on Probiotic Activity of Bifidobacterium animalis ssp. lactis BB-12®
Andreja Leboš Pavunc
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Lenkica Penava
; Belupo, Pharmaceuticals & Cosmetics Inc., Nutraceuticals, Business Development and Registration, I. Savica 36, 10000 Zagreb, Croatia
Jasmina Ranilović
; Podravka, Research and Development, Ante Starčevića 32, 48000 Koprivnica, Croatia
Jasna Novak
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Martina Banić
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Katarina Butorac
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Emilija Petrović
; Podravka, Research and Development, Ante Starčevića 32, 48000 Koprivnica, Croatia
Vesna Mihaljević-Herman
; Podravka, Research and Development, Ante Starčevića 32, 48000 Koprivnica, Croatia
Krešo Bendelja
; University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10000 Zagreb, Croatia
Ana Savić Mlakar
; University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10000 Zagreb, Croatia
Ksenija Durgo
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for Biology and Microbial Genetics, Pierottijeva 6, 10000 Zagreb, Croatia
Blaženka Kos
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Jagoda Šušković
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, Pierottijeva 6, 10000 Zagreb, Croatia
Sažetak
Three novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12® (BB-12®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12®.
Ključne riječi
Bifidobacterium; probiotics; dehydrated wheat/rice cereal products
Hrčak ID:
223856
URI
Datum izdavanja:
30.6.2019.
Posjeta: 1.806 *