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Agriculturae Conspectus Scientificus, Vol. 84 No. 3, 2019.

Izvorni znanstveni članak

Genetic Diversity of zyxin and TNFRSF1A genes in Nigerian Local Chickens and Nera Black Chickens

Adeyemi Sunday Adenaike orcid id orcid.org/ORCID ; Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria. P.M.B. 2240 Abeokuta, Nigeria
Sunday Olusola Peters orcid id orcid.org/ORCID ; Department of Animal Science, Berry College, Mount Berry, GA, 30149, USA
Adeboye Olusesan Fafiolu orcid id orcid.org/ORCID ; Department of Animal Nutrition, Federal University of Agriculture, Abeokuta, Nigeria
Matthew Adeleke Adeleke orcid id orcid.org/ORCID ; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), P/ Bag X54001 Durban 4000, South Africa
Micheal Irewole Takeet orcid id orcid.org/ORCID ; Department of Veterinary Microbiology and Parasitology, Federal university of Agriculture, Abeokuta, Nigeria
Mathew Wheto orcid id orcid.org/ORCID ; Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria. P.M.B. 2240 Abeokuta, Nigeria
Olufunmilayo Ayoka Adebambo orcid id orcid.org/ORCID ; Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria. P.M.B. 2240 Abeokuta, Nigeria
Christian Obiora N. Ikeobi orcid id orcid.org/ORCID ; Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Nigeria. P.M.B. 2240 Abeokuta, Nigeria

Puni tekst: engleski pdf 561 Kb

str. 305-311

preuzimanja: 235

citiraj

Sažetak

This study was conducted to assess the population and genetic diversity of TNFRSF1A and zyxin genes among the Nigerian indigenous (Naked neck, Normal  and  Frizzle  feather) and Nera Black chickens (exotic chicken). Blood samples were collected from 400 chickens belonging to the genotypes described above and genomic DNA extracted using commercially available kit. Genomic regions containing zyxin and TNFRSF1A genes were amplified, sequenced and analyzed using Bioedit, DnaSP, MEGA and PANTHER software. The results from analysis revealed that Naked neck and Normal feather chickens had the highest mean value of nucleotide substitutions per sites (Dxy) (0.081), while Frizzle and Nera Black local chickens had the lowest Dxy value of 0.065 for TNFRSF1A gene sequences. The results from the analysis of zyxin gene sequence revealed that Frizzle and Normal feather chickens had the highest Dxy (0.6551), while Normal feather and Naked neck chickens had the lowest Dxy (0.0739). Nera Black chickens TNFRSF1A gene sequence had the highest mean values of haplotype diversity and average number of nucleotide difference (0.923 and 3.967 respectively). In zyxin sequences, the highest average number of nucleotide difference (3.143) and nucleotide diversity (0.00489) were detected in the Frizzle feather chicken. The analyses of these two genes sequences revealed high nucleotide divergence and haplotypes diversity. The results of divergence, haplotypes diversity and phylogenetic tree indicated a historically restricted gene flow among the genotypes.

Ključne riječi

Genetic diversity; TNFSFR1A gene; zyxin gene; Indigenous chickens

Hrčak ID:

225544

URI

https://hrcak.srce.hr/225544

Datum izdavanja:

24.9.2019.

Posjeta: 834 *