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https://doi.org/10.5513/JCEA01/20.4.2294

The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen

Michal Duračka   ORCID icon orcid.org/0000-0002-2907-5909 ; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic
Megane Debacker ; Condorcet - Hainaut Provincial High School, Tournai, Belgium
Ondrej Bučko ; Department of Animal Husbandry, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Slovak Republic
Norbert Lukač ; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic
Eva Tvrda ; Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Slovak Republic

Puni tekst: engleski, pdf (868 KB) str. 1069-1075 preuzimanja: 127* citiraj
APA 6th Edition
Duračka, M., Debacker, M., Bučko, O., Lukač, N. i Tvrda, E. (2019). The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen . Journal of Central European Agriculture, 20 (4), 1069-1075. https://doi.org/10.5513/JCEA01/20.4.2294
MLA 8th Edition
Duračka, Michal, et al. "The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen ." Journal of Central European Agriculture, vol. 20, br. 4, 2019, str. 1069-1075. https://doi.org/10.5513/JCEA01/20.4.2294. Citirano 20.10.2021.
Chicago 17th Edition
Duračka, Michal, Megane Debacker, Ondrej Bučko, Norbert Lukač i Eva Tvrda. "The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen ." Journal of Central European Agriculture 20, br. 4 (2019): 1069-1075. https://doi.org/10.5513/JCEA01/20.4.2294
Harvard
Duračka, M., et al. (2019). 'The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen ', Journal of Central European Agriculture, 20(4), str. 1069-1075. https://doi.org/10.5513/JCEA01/20.4.2294
Vancouver
Duračka M, Debacker M, Bučko O, Lukač N, Tvrda E. The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen . Journal of Central European Agriculture [Internet]. 2019 [pristupljeno 20.10.2021.];20(4):1069-1075. https://doi.org/10.5513/JCEA01/20.4.2294
IEEE
M. Duračka, M. Debacker, O. Bučko, N. Lukač i E. Tvrda, "The effect of kaempferol and naringenin may improve the in vitro quality of stored boar semen ", Journal of Central European Agriculture, vol.20, br. 4, str. 1069-1075, 2019. [Online]. https://doi.org/10.5513/JCEA01/20.4.2294

Sažetak
Determination of the effect of pure bioactive substances on male reproductive cells is a current theme amongst veterinary andrologists. The aim of this study was to investigate the in vitro effect of various concentrations of kaempferol and naringenin on boar male gametes in different time periods (0, 24, 48 and 72h). The computer-assisted semen analysis was used to measure the sperm motility. Mitochondrial activity was determined by the mitochondrial toxicity test (MTT). The formation of the superoxide radical, as a marker of oxidative stress, was analysed by the nitroblue-tetrazolium (NBT) test. The results showed that every selected concentration of both substances came along with beneficial effect on the structure and function of spermatozoa. Particularly, naringenin at concentrations of 10-50 µmol/L and kaempferol at concentrations of 5-25 µmol/L may provide beneficial effects on spermatozoa, thus prolong the storage period and improve the quality of preserved boar spermatozoa.

Ključne riječi
antioxidants; bioactive compounds; kaempferol; naringenin; sperm; spermatozoa; oxidative stress; reactive oxygen species; mitochondrial activity

Hrčak ID: 230867

URI
https://hrcak.srce.hr/230867

[slovački]

Posjeta: 313 *