Izvorni znanstveni članak
Use of ELISA for detection of Trichinella spiralis during veterinary-sanitary inspection of meat of pigs
Josip Barić
Bela Njari
; Zavod za higijenu i tehnologiju animalnih namirnica, Veterinarski fakultet Sveučilišta u Zagrebu, Heinzelova 55, Zagreb
Albert Marinculić
; Zavod za parazitologiju i invazijske bolesti, Veterinarski fakultet Sveučilišta u Zagrebu, Heinzelova 55, Zagreb
Relja Beck
; Zavod za parazitologiju i invazijske bolesti, Veterinarski fakultet Sveučilišta u Zagrebu, Heinzelova 55, Zagreb
Sažetak
Epizootiological situation with trichinellosis of pigs in the Republic of Croatia, especially in its Eastern counties, is not favourable. The currently prescribed officialmethod of classical trichinelloscopy, with respect to its sensibility, can prevent the infection of people, but it is definitely insufficient for an efficient prevention of the spreading chain of trichinellosis. ELISA, as a more sensitive method of inspection, has to find its place in epidemiological researches and monitoring of pig breeding in some regions of Croatia, as well as in individual farm breeding, in order to determine the epizootiological status of the pig population that is being monitored. In this way it would be possible to separate all suspicious pigs while alive (positive, false-positive, but false- negative, too), and the remaining part of the population could be then slaughtered in the registered slaughter-houses, as well as on family farms, without fear that the infected pigs would be overlooked because it is beyond the detecting possibilities of classical trichinelloscopy.
The introduction of ELISA on a range of pig slaughters as the only method of inspection is not possible, firstlybecause of the occurrence of a smaller number of falsely negative animals, which, in epidemiological sense, represent a great risk in the spreading chain of this disease, which can lead in certain circumstances to infection of people.
Ključne riječi
ELISA; trichinellosis; diagnostic
Hrčak ID:
21969
URI
Datum izdavanja:
15.4.2007.
Posjeta: 2.213 *