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https://doi.org/10.20471/acc.2020.59.03.02

Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats

Ivan Mirković   ORCID icon orcid.org/0000-0002-9462-2067 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Tamara Kralj ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Marin Lozić   ORCID icon orcid.org/0000-0001-5915-626X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Vasilije Stambolija   ORCID icon orcid.org/0000-0003-0670-3965 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Josip Kovačević   ORCID icon orcid.org/0000-0001-7426-201X ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Luka Vrdoljak ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Mirna Zlatar ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Kristina Milanović ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Domagoj Drmić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Jurica Predović   ORCID icon orcid.org/0000-0002-4399-3140 ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sanja Masnec ; Department of Ophthalmology, Zagreb University Hospital Centre, Zagreb, Croatia
Matija Jurjević ; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Mladen Bušić ; Department of Ophthalmology, Sveti Duh University Hospital, Zagreb, Croatia
Sven Seiwerth ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia
Antonio Kokot   ORCID icon orcid.org/0000-0002-4836-7536 ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Anatomy and Neuroscience, Osijek Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Predrag Sikirić ; Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb, Croatia

Puni tekst: engleski, pdf (637 KB) str. 394-406 preuzimanja: 143* citiraj
APA 6th Edition
Mirković, I., Kralj, T., Lozić, M., Stambolija, V., Kovačević, J., Vrdoljak, L., ... Sikirić, P. (2020). Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats. Acta clinica Croatica, 59. (3.), 394-406. https://doi.org/10.20471/acc.2020.59.03.02
MLA 8th Edition
Mirković, Ivan, et al. "Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats." Acta clinica Croatica, vol. 59., br. 3., 2020, str. 394-406. https://doi.org/10.20471/acc.2020.59.03.02. Citirano 08.12.2021.
Chicago 17th Edition
Mirković, Ivan, Tamara Kralj, Marin Lozić, Vasilije Stambolija, Josip Kovačević, Luka Vrdoljak, Mirna Zlatar, et al. "Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats." Acta clinica Croatica 59., br. 3. (2020): 394-406. https://doi.org/10.20471/acc.2020.59.03.02
Harvard
Mirković, I., et al. (2020). 'Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats', Acta clinica Croatica, 59.(3.), str. 394-406. https://doi.org/10.20471/acc.2020.59.03.02
Vancouver
Mirković I, Kralj T, Lozić M, Stambolija V, Kovačević J, Vrdoljak L i sur. Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats. Acta clinica Croatica [Internet]. 2020 [pristupljeno 08.12.2021.];59.(3.):394-406. https://doi.org/10.20471/acc.2020.59.03.02
IEEE
I. Mirković, et al., "Pentadecapeptide BPC 157 Shortens Duration of Tetracaine- and Oxybuprocaine-Induced Corneal Anesthesia in Rats", Acta clinica Croatica, vol.59., br. 3., str. 394-406, 2020. [Online]. https://doi.org/10.20471/acc.2020.59.03.02

Sažetak
We focused on the relationship of 0.5% tetracaine- and 0.4% oxybuprocaine-induced corneal anesthesia in rats, and pentadecapeptide BPC 157 (0.4 μg/eye), along with nitric oxide synthase (NOS) inhibitor N(gamma)-nitro-L-arginine methyl ester (L-NAME) (0.1 mg/eye) and/or NOS substrate L-arginine (2 mg/eye), applied in the form of eye drops. We assessed corneal sensitivity recovery (Cochet-Bonnet esthesiometer), corneal lesion elimination (staining with 10% fluorescein) and decrease in tear volume (Schirmer test). BPC 157 administration had a full counteracting effect. Recovery also occurred in the presence of NOS blockade and NOS substrate application. L-arginine eventually shortened duration of corneal insensitivity and exerted corneal lesion counteraction (and counteraction of tetracaine-induced decrease of tear volume) only in earlier but not in later period. L-NAME application led to longer duration of corneal insensitivity, increase in corneal lesions and decrease in tear volume. When L-NAME and L-arginine were applied together, they antagonized each other’s effect. These distinctions may indicate particular NOS involvement (corneal insensitivity vs. corneal lesion along with tear production), distinctively affected by the administration of NO agents. However, additional BPC 157 co-administration would re-establish counteraction over topical ophthalmic anesthetic-induced effect, be it in its early or late course. We suggest BPC 157 as an antidote to topical ophthalmic anesthetics.

Ključne riječi
BPC 157; Tetracaine; Oxybuprocaine; Corneal anesthesia; NOS; Rats

Hrčak ID: 247347

URI
https://hrcak.srce.hr/247347

[hrvatski]

Posjeta: 406 *