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Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses

Marina Korolija ; Ruđer Bošković Institute, Zagreb, Croatia
Mirko Hadžija ; Ruđer Bošković Institute, Zagreb, Croatia
Pajica Pavković ; Vuk Vrhovac University Clinic, Zagreb, Croatia
Sanja Kapitanović ; Ruđer Bošković Institute, Zagreb, Croatia
Marijana Popović Hadžija ; Ruđer Bošković Institute, Zagreb, Croatia

Puni tekst: engleski, pdf (96 KB) str. 437-441 preuzimanja: 990* citiraj
APA 6th Edition
Korolija, M., Hadžija, M., Pavković, P., Kapitanović, S. i Popović Hadžija, M. (2008). Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses. Croatica Chemica Acta, 81 (3), 437-441. Preuzeto s https://hrcak.srce.hr/31128
MLA 8th Edition
Korolija, Marina, et al. "Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses." Croatica Chemica Acta, vol. 81, br. 3, 2008, str. 437-441. https://hrcak.srce.hr/31128. Citirano 21.11.2019.
Chicago 17th Edition
Korolija, Marina, Mirko Hadžija, Pajica Pavković, Sanja Kapitanović i Marijana Popović Hadžija. "Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses." Croatica Chemica Acta 81, br. 3 (2008): 437-441. https://hrcak.srce.hr/31128
Harvard
Korolija, M., et al. (2008). 'Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses', Croatica Chemica Acta, 81(3), str. 437-441. Preuzeto s: https://hrcak.srce.hr/31128 (Datum pristupa: 21.11.2019.)
Vancouver
Korolija M, Hadžija M, Pavković P, Kapitanović S, Popović Hadžija M. Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses. Croatica Chemica Acta [Internet]. 2008 [pristupljeno 21.11.2019.];81(3):437-441. Dostupno na: https://hrcak.srce.hr/31128
IEEE
M. Korolija, M. Hadžija, P. Pavković, S. Kapitanović i M. Popović Hadžija, "Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses", Croatica Chemica Acta, vol.81, br. 3, str. 437-441, 2008. [Online]. Dostupno na: https://hrcak.srce.hr/31128. [Citirano: 21.11.2019.]

Sažetak
The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity
using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue
were tested. Elctrophoresis of amplified loci was performed on a standard acrylamide / N,Nmethylenebisacrylamide
gel, and the same gel containing Spreadex polymer, NAB (native acrylamide-
bis). Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis
of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples.
Five of 12 matched tumor samples displayed loss of heterozygosity that could not be detected
on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents
a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity
detection, with improved performances such as increased sensitivity, decreased amount of a
sample and improved separation of closely spaced bands. Application of the polymer may contribute
to a vide variety of diagnostic procedures.

Ključne riječi
DPC4; loss of heterozygosity; allele; Spreadex polymer NAB

Hrčak ID: 31128

URI
https://hrcak.srce.hr/31128

Posjeta: 1.203 *