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Aminopeptidases of Germinated and Non-Germinated Barley

Ivica Strelec   ORCID icon orcid.org/0000-0003-3999-8499 ; Laboratory of Biochemistry, Faculty of Food Technology, Franje Kuhača 18, HR-31000 Osijek, Croatia
Bojana Vukelić ; Laboratory of Cellular Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Ljubinka Vitale ; Laboratory of Cellular Biochemistry, Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (171 KB) str. 296-303 preuzimanja: 319* citiraj
APA 6th Edition
Strelec, I., Vukelić, B. i Vitale, Lj. (2009). Aminopeptidases of Germinated and Non-Germinated Barley. Food Technology and Biotechnology, 47 (3), 296-303. Preuzeto s https://hrcak.srce.hr/39847
MLA 8th Edition
Strelec, Ivica, et al. "Aminopeptidases of Germinated and Non-Germinated Barley." Food Technology and Biotechnology, vol. 47, br. 3, 2009, str. 296-303. https://hrcak.srce.hr/39847. Citirano 20.09.2019.
Chicago 17th Edition
Strelec, Ivica, Bojana Vukelić i Ljubinka Vitale. "Aminopeptidases of Germinated and Non-Germinated Barley." Food Technology and Biotechnology 47, br. 3 (2009): 296-303. https://hrcak.srce.hr/39847
Harvard
Strelec, I., Vukelić, B., i Vitale, Lj. (2009). 'Aminopeptidases of Germinated and Non-Germinated Barley', Food Technology and Biotechnology, 47(3), str. 296-303. Preuzeto s: https://hrcak.srce.hr/39847 (Datum pristupa: 20.09.2019.)
Vancouver
Strelec I, Vukelić B, Vitale Lj. Aminopeptidases of Germinated and Non-Germinated Barley. Food Technology and Biotechnology [Internet]. 2009 [pristupljeno 20.09.2019.];47(3):296-303. Dostupno na: https://hrcak.srce.hr/39847
IEEE
I. Strelec, B. Vukelić i Lj. Vitale, "Aminopeptidases of Germinated and Non-Germinated Barley", Food Technology and Biotechnology, vol.47, br. 3, str. 296-303, 2009. [Online]. Dostupno na: https://hrcak.srce.hr/39847. [Citirano: 20.09.2019.]

Sažetak
In processes of barley plant development, various endo- and exopeptidases are involved. To determine the type and number of aminopeptidases that could participate in barley seed germination and tissue growth, their activities in extracts of non-germinated and germinated barley (Hordeum vulgare L. cv. Angora) grains and young tissues have been examined, and some of their properties determined. Aminopeptidases (AP) hydrolysing 2-naphthylamides of various amino acids were present in dry and germinated grains, roots, seedlings and leaves, showing preferences for amino acids phenylalanine (Phe), arginine (Arg), leucine (Leu) and methionine (Met), and lower activity towards alanine (Ala), proline (Pro), glycine (Gly) and histidine (His). Levels and ratios of AP activities changed during germination and tissue development, indicating that APs of different specificities are required at different stages of germination and in young tissues. Thus, the increase of all aminopeptidase activities during the first 24 hours of germination and subsequent decrease show significant involvement in seed primary metabolism restoration. The activities of Arg- and HisAP are equally important in green malt. Seedlings and leaves have pronounced substrate specificity for Phe, Leu, Ala and Pro, while roots have the lowest AP specific activities. From the activities and determined properties, the presence of at least six aminopeptidases optimally active at pH=7.4–8.2 could be discerned in dry and germinated grains, and young tissues of Angora barley. Two aminopeptidases are most probably of broad substrate specificity, three show narrow preference with dominating Leu, Phe, or Pro/His, while one is specific for Arg.

Ključne riječi
aminopeptidase activities; barley; germination; gel filtration; isoelectric focusing (IEF)

Hrčak ID: 39847

URI
https://hrcak.srce.hr/39847

[hrvatski]

Posjeta: 645 *