Izvorni znanstveni članak
https://doi.org/10.17113/ftb.54.01.16.4256
Dose-Dependent Protective and Inductive Effects of Xanthohumol on Oxidative DNA Damage in Saccharomyces cerevisiae
Daniel O. Carvalho
orcid.org/0000-0002-4459-7908
; REQUIMTE/LAQV – Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Porto, Portugal
Rui Oliveira
; Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Department of Biology, University of Minho, Braga, Portugal
Björn Johansson
; Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal
Luís F. Guido
orcid.org/0000-0001-9441-7867
; REQUIMTE/LAQV – Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Porto, Portugal
Sažetak
The effect of xanthohumol, a prenylflavonoid isolated from the hop plant (Humulus lupulus L.), on Saccharomyces cerevisiae DNA oxidative damage and viability was evaluated. Yeast cultures under oxidative stress, induced by H2O2, displayed stronger growth in the presence of 5 mg/L of xanthohumol than cultures with only H2O2. Likewise, DNA damage assessed by the comet assay was significantly lower in cells co-incubated with xanthohumol and H2O2. Accordingly, fluorescence of dichlorofluorescein in cells treated with H2O2 and xanthohumol was considerably lower than in cells exclusively treated with H2O2, indicative of a reactive oxygen species scavenging mechanism and consequent formation of oxidation products, as detected by mass spectrometry. However, at concentrations above 5 mg/L, xanthohumol elicited an opposite effect, leading to a slower growth rate and significant increase in DNA damage. A yeast yap1 deletion mutant strain sensitive to oxidative stress grew more slowly in the presence of at least 5 mg/L of xanthohumol than cultures of the wild type, suggesting that xanthohumol toxicity is mediated by oxidative stress. This evidence provides further insight into the impact of xanthohumol on yeast cells, supporting dose-dependent antioxidant/antigenotoxic and prooxidant/genotoxic effects.
Ključne riječi
genotoxic and antigenotoxic effects; antioxidant and prooxidant capacity; comet assay; Saccharomyces cerevisiae; xanthohumol; yeast viability
Hrčak ID:
155093
URI
Datum izdavanja:
31.3.2016.
Posjeta: 1.356 *