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Primary proteolysis of white brined goat cheese monitored by high molarity Tris buffer SDS- PAGE system

Miroljub B. Barać orcid id ; Institute of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11081 Belgrade, Serbia
Milenko Smiljanić
Mirjana B. Pešić
Sladjana P. Stanojević
Snežana T. Jovanović
Ognjen D. Maćej

Puni tekst: engleski pdf 436 Kb


str. 122-131

preuzimanja: 1.506



The aim of this work was to investigate primary proteolysis of white brined goat cheese prepared from raw milk and to correlate with the results obtained with high-molarity Tris buffer electrophoretic system. Proteolytic changes of white brined cheese were monitored by three parameters, the total protein, the water soluble proteins and the degree of proteolysis, whereas the change of major casein fractions was followed by electrophoresis in reducing conditions. Ripening caused a decrease of the total protein content, whereas in the first forty days the water soluble protein and the degree of proteolysis increased. Both casein fractions (αs- and β-casein) of goat cheese were susceptible to primary proteolysis but to the different extent. αs1- casein disappeared during processing, whereas during 60-days of ripening the content of αs2 and β-casein was reduced by 38.90 %, and 30.72 %, respectively. Such trends of major casein fractions were strongly correlated with the degree of proteolysis and the moisture content, whereas no correlation was found between them and the water soluble protein content. The results of our investigation clearly suggested that SDS-PAGE method based on high-molarity Tris-buffer system could be a very useful for purposes of monitoring the white goat cheese proteolysis.

Ključne riječi

proteolysis; SDS-PAGE; goat cheese

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Podaci na drugim jezicima: hrvatski

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