Original scientific paper

Basing a selective method for isolating environmental Vibrio cholerae on differences in the growth rate of competing Vibrio metschnikovii.

Vladimir Muić ; Croatian National Institute of Public Health, Zagreb, Croatia
Mate Ljubičić ; Croatian National Institute of Public Health, Zagreb, Croatia
Ivan Vodopija ; Zagreb Public Health Institute, Zagreb, Croatia
Vladimir Mayer ; Croatian National Institute of Public Health, Zagreb, Croatia

Abstract

Some differential growth characteristics for Vibrio cholerae and Vibrio metschnikovii were looked at in a peptone water vibrio enrichment basic liquid medium by varying pH, ionic strength, as well as temperature and length of incubation. The purpose was to separate the two bacterial species, i.e. to establish which combination of the above cultivation conditions might enable a selective replication of V. cholerae (human and animal pathogen), while suppressing the growth of V. metschnikovii. The latter vibrio hinders visual identification of the V. cholerae of waste water and animal origin on a solid TCBS standard selective medium. A preliminary limited routine verification of potential methods, as applied to waste water samples, showed that a combination of cultivation conditions which involved an extension of incubation to 22 h at a heightened temperature of 41 °C might enable Vibrio cholerae to be simply and rapidly isolated, with visual inspection already providing its final identification and quantification.

Keywords

selective isolation; Vibrio cholerae; Vibrio metschnikovii

Hrčak ID:

109097

URI

https://hrcak.srce.hr/109097

Publication date:

23.6.1999.

Article data in other languages: croatian

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