Izvorni znanstveni članak
Improvement of Cell Wall Degrading Enzymes Production by Alginate Encapsulated Trichoderma spp.
Momein H. El-Katatny
; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Ahmed M. Hetta
; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Gehan M. Shaban
; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Hesham M. El-Komy
; Department of Botany, Faculty of Science, El-Minia University, El-Minia, 61519, Egypt
Sažetak
Conidia of three Trichoderma isolates were formulated to make alginate pellets with or without 0.5 % chitin or dried fungal mycelium of Fusarium oxysporum as carbon source. The formulations were compared for their ability of in vitro chitinase and β-1,3-glucanase
production with free fungal spore suspensions. Conidia entrapped in alginate with or without adjuvant showed high production of enzymes (especially for chitinase) even when repeated 4 times. The addition of chitin or dried fungal mycelium as adjuvant enhanced the enzyme production up to 5 and 2-fold for chitinase and β-1,3-glucanase, respectively. Alginate concentration and surface area of the beads affected the enzyme production. The optimum initial pH, incubation time and temperature were pH=6, 12 days and 40 °C for chitinase, and pH=7, 10 days and 35 °C for β-1,3-glucanase production. The improvement of cell wall degrading enzyme production by alginate encapsulated Trichoderma could explain the in vivo inhibitory effect of such formulations on the target phytopathogenic fungi.
Ključne riječi
Trichoderma spp.; biocontrol; chitinase; glucanase, immobilization; Ca-alginate
Hrčak ID:
118617
URI
Datum izdavanja:
15.9.2003.
Posjeta: 1.505 *