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FIRST MOLECULAR DETECTION OF CROATIAN POTATO CYST NEMATODE (PCN) POPULATIONS USING THE POLYMERASE CHAIN REACTION (PCR)

Dinka Grubišić orcid id orcid.org/0000-0001-5722-7917 ; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Ivana Pajač Živković ; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Tanja Gotlin Čuljak ; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Mirjana Brmež ; Poljoprivredni fakultet u Osijeku, Zavod za zaštitu bilja, Osijek, Hrvatska
Teuta Benković-Lačić ; Veleučilište u Slavonskom Brodu, Slavonski Brod, Hrvatska
Aleksandar Mešić ; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska


Puni tekst: engleski pdf 382 Kb

str. 35-40

preuzimanja: 493

citiraj


Sažetak

The potato cyst nematodes (PCN), Globodera rostochiensis and Globodera pallida are economically important pests of the potato (Solanum tuberosum L. subsp. tuberosum) and are recognized as quarantine pests internationally. In Croatia, PCN were discovered for the first time in Međimurska county in 2001. Since then all identifications of PCN populations for monitoring purposes have
been based on morphological characters. In order to introduce the use of the precise and reliable molecular method of PCN identification, the polymerase chain reaction (PCR) was conducted, for the first time in Croatia. Another aim of this investigation was to find out the composition of PCN populations in Croatia. For this purpose ten PCN populations from three Croatian counties (Međimurska, Varaždinska, Primorsko-goranska) were analysed. All populations in investigation were identified as G. rostochiensis.

Ključne riječi

Croatia; diagnostics; Globodera rostochiensis; Globodera pallida; PCR; potato cyst nematodes

Hrčak ID:

130292

URI

https://hrcak.srce.hr/130292

Datum izdavanja:

9.12.2013.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.126 *