Izvorni znanstveni članak
Interaction between Human Serum Albumin and Different Anatase TiO2 Nanoparticles: A Nano-bio Interface Study
Viviana Vergaro
; CNR NANOTEC, Institute of Nanotechnology of the National Research Council, c/o Campus Ecotekne, University of Salento, Lecce, Italy
Claudia Carlucci
; Chemistry Department, University of Bari, Bari, Italy
Mariafrancesca Cascione
; CNR NANOTEC, Institute of Nanotechnology of the National Research Council, c/o Campus Ecotekne, University of Salento, Lecce, Italy; Mathematics and Physics Department “Ennio De Giorgi”, University of Salento, Lecce, Italy
Caterina Lorusso
; Innovation Engineering Department, University of Salento, Lecce, Italy
Francesca Conciauro
; Innovation Engineering Department, University of Salento, Lecce, Italy
Barbara Federica Scremin
; CNR NANOTEC, Institute of Nanotechnology of the National Research Council, c/o Campus Ecotekne, University of Salento, Lecce, Italy
Paola Maria Congedo
; Innovation Engineering Department, University of Salento, Lecce, Italy
Giuseppe Cannazza
; Life Sciences Department, University of Modena and Reggio Emilia, Modena, Italy
Cinzia Citti
; Biology and Environmental Sciences Department, University of Salento, Lecce, Italy
Giuseppe Ciccarella
orcid.org/0000-0003-4467-0922
; CNR NANOTEC, Institute of Nanotechnology of the National Research Council, c/o Campus Ecotekne, University of Salento, Lecce, Italy; Innovation Engineering Department, University of Salento, Lecce, Italy
Sažetak
In this investigation, differently shaped and surface functionalized TiO2 anatase nanoparticles and human serum albumin (HSA) were selected to study protein-nanoparticles interaction both in a solution and on flat surfaces, thereby mimicking a medical device. Anatase nanocrystals were characterized by transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) surface analysis and dynamic light scattering (DLS). The protein-nanoparticles’ interactions and their eventual reversibility were studied by pH dependent ζ- potential measurements in different media: ultra-pure water, a phosphate buffer simulating physiological conditions and in a culture medium supplemented with foetal bovine serum. The protein corona masking effect was evidenced and the interaction HSA-nanocrystals resulted irreversible. The interaction HSA-silicon supported TiO2 nanocrystals films was studied by atomic force microscopy (AFM), and resulted driven by the substrate hydrophilicity degree plus was different for the diverse range of nanocrystals tested. Surface roughness measurements showed that on some of the nanocrystals, HSA were arranged in a more globular manner. A lower protein affinity was found for nanocrystals that had a smaller primary particle size, which may correspond to their higher biocompatibility. This nano-bio interface research aimed to study the HSA protein-TiO2 anatase nanocrystals under conditions similar to those for in vitro and in vivo toxicity analyses.
Ključne riječi
TiO2; Nanocrystals; Albumin; Interface; Adhesion; ζ-potential
Hrčak ID:
157554
URI
Datum izdavanja:
1.1.2015.
Posjeta: 1.309 *