Acta Botanica Croatica, Vol. 58 No. 1, 1999.
Izvorni znanstveni članak
Molecular structure of spinach chloroplast nucleoids
Hrvoje Fulgosi
; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Nikola Ljubešić
; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Sažetak
The ultrastructure and molecular composition of spinach chloroplast nucleoids were studied. Isolated nucleoids retained their three-dimensionally folded structure observed in situ. Fluorescence microscopy and staining with DAP1 reaffirmed the mostly DNA-containing nature of these complexes. The presence of chloroplast DNA was confirmed by restriction fragment analysis. Electron microscopy revealed a highly condensed, chromatin-like, beaded substructure. Naked DNA supercoils or loops were not observed. Integral nucleoid proteins were isolated and analysed on SDS-polyacrylamide gels. A distinct pattern of the nucleoid proteins, covering the entire range of molecular masses from 5 kDa to >100 kDa revealed that the nucleoid fraction was highly depleted of major thylakoid and stromal constituents.
Ključne riječi
spinach; chloroplast nucleoides; cpDNA; proteins; microscopy
Hrčak ID:
160581
URI
Datum izdavanja:
31.12.1999.
Posjeta: 1.331 *