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Effects of abscisic acid and carbohydrates on the maturation of Picea omorika (Panč.) Purk. somatic embryos

Tamara Tramišak-Milaković ; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Snježana Mihaljević ; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Sibila Jelaska ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia


Puni tekst: engleski pdf 576 Kb

str. 87-97

preuzimanja: 303

citiraj


Sažetak

In eight embryogenie tissue lines of omorika spruce the roles of carbohydrates and abscisic acid on embryo maturation were examined. Sucrose, glucose and maltose (3% and 6%) with abscisic acid (20, 40, 60 μM) were screened for optimal combination with respect to embryo maturation. The B lines, which expressed a brown tissue phenotype, achieved superior embryo maturation on 3% glucose and 40 μM abscisic acid. The VV lines with a white and translucent tissue phenotype showed the best maturation capacity on 3% sucrose or 3% maltose with 20 or 40 μM ABA. Sixty μM ABA inhibited embryo maturation. Media containing 6 % of either sucrose, glucose and maltose produced fewer mature embryos compared to media with 3% of the same sugars. The VV lines had almost three times lower maturation capacity in relation to the B lines.

Ključne riječi

Picea omorika; omorika spruce; somatic embryogenesis; abscisic acid; embryo maturation; carbohydrates

Hrčak ID:

160606

URI

https://hrcak.srce.hr/160606

Datum izdavanja:

31.12.1999.

Posjeta: 810 *