Acta Botanica Croatica, Vol. 66 No. 2, 2007.
Izvorni znanstveni članak
Sugar-induced changes in cellular and extracellular protein and glycoprotein patterns of sugarbeet cell lines
Dubravko Pavoković
orcid.org/0000-0001-7198-6063
; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10000 Zagreb, Croatia
Ivana Šola
; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10000 Zagreb, Croatia
Daniel Hagège
; Département de Biologie, UFR Faculté des Sciences, Université d’Orléans, Rue de Chartres, BP 6759, 45067 Orléans Cedex 2, France
Marijana Krsnik-Rasol
; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10000 Zagreb, Croatia
Sažetak
Plants sense carbohydrates and transduce a signal which changes gene expression and the activities of many enzymes. The relationship between changes in gene expression by carbohydrates and the developmental state of the cells is still poorly understood. To gain more knowledge about this relationship, we have analyzed three sugar beet (Beta vulgaris L.) in vitro cell lines residing on distinct differentiation states. Cell suspensions were initiated and cells were incubated for 72h in the medium with sucrose as a control, or treated
during the same period with glucose or 3-O-methylglucose (3OMG). Cellular and extracellular proteins, separated electrophoretically, showed that sugar-induced protein expression was cell line-specific. More differences were visible in extracellular and in glycoprotein than on cellular protein patterns. The 3OMG downregulated while glucose
upregulated cellular glycoproteins. In the case of extracellular proteins, glucose and 3OMG were equally effective in both downregulation and upregulation of protein expression. Sialic acid was indicated as a glycan conjugate in sugar beet. Carbohydrate-induced
gene expression was related to the developmental state of cells.
Ključne riječi
cell differentiation; glycoproteins; SDS-PAGE; sugar beet; sugar sensing
Hrčak ID:
17180
URI
Datum izdavanja:
20.10.2007.
Posjeta: 2.033 *