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Original scientific paper

SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE

Dubravka Vitali Čepo ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Petra Albahari ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Marijana Zovko Končić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Kristina Radić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Sanja Jurmanović ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Mario Jug ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia

Fulltext: english, pdf (702 KB) pages 7-14 downloads: 1.260* cite
APA 6th Edition
Vitali Čepo, D., Albahari, P., Zovko Končić, M., Radić, K., Jurmanović, S. & Jug, M. (2017). SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE. Hrana u zdravlju i bolesti, 6 (1), 7-14. Retrieved from https://hrcak.srce.hr/182916
MLA 8th Edition
Vitali Čepo, Dubravka, et al. "SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE." Hrana u zdravlju i bolesti, vol. 6, no. 1, 2017, pp. 7-14. https://hrcak.srce.hr/182916. Accessed 27 Sep. 2021.
Chicago 17th Edition
Vitali Čepo, Dubravka, Petra Albahari, Marijana Zovko Končić, Kristina Radić, Sanja Jurmanović and Mario Jug. "SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE." Hrana u zdravlju i bolesti 6, no. 1 (2017): 7-14. https://hrcak.srce.hr/182916
Harvard
Vitali Čepo, D., et al. (2017). 'SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE', Hrana u zdravlju i bolesti, 6(1), pp. 7-14. Available at: https://hrcak.srce.hr/182916 (Accessed 27 September 2021)
Vancouver
Vitali Čepo D, Albahari P, Zovko Končić M, Radić K, Jurmanović S, Jug M. SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE. Hrana u zdravlju i bolesti [Internet]. 2017 [cited 2021 September 27];6(1):7-14. Available from: https://hrcak.srce.hr/182916
IEEE
D. Vitali Čepo, P. Albahari, M. Zovko Končić, K. Radić, S. Jurmanović and M. Jug, "SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE", Hrana u zdravlju i bolesti, vol.6, no. 1, pp. 7-14, 2017. [Online]. Available: https://hrcak.srce.hr/182916. [Accessed: 27 September 2021]

Abstracts
Olive oil mill waste obtained after two-phase olive oil extraction process was subjected to conventional liquid solvent extraction under different pH/temperature/duration conditions using different types of food-grade solvents. The independent variables were: solvent type (ethanol percentage), extraction temperature (20-90 °C), extraction time (30 min-24 h) and pH (2-10.3) of extraction solvent while the response variables were total phenolic content and antioxidant activity of obtained extracts. The optimum solvent extraction conditions for phenols were 120 min at 70 °C using 60% ethanol as extraction solvent, at solvent to sample ratio 5:1 (v/w). For quantification of major bioactive olive polyphenols hydroxytyrosol, tyrosol and oleuropein in obtained extracts, fast and simple RP-HPLC-DAD method was developed and validated. Oleuropein was presented in highest amounts with average value of 115.14±0.19 mg/kg of fresh olive pomace.

Keywords
olive pomace; extraction; HPLC-DAD; hydroxytyrosol; tyrosol; oleuropein

Hrčak ID: 182916

URI
https://hrcak.srce.hr/182916

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