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Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers

Novak Mario   ORCID icon orcid.org/0000-0002-9638-615X ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Mladen Pavlečić ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Baghish Harutyunyan ; The Scientific Production Center "Armbiotechnology" of the National Academy of Sciences of the Republic of Armenia, Yerevan, Republick of Armenia
Vigen Goginyan ; The Scientific Production Center "Armbiotechnology" of the National Academy of Sciences of the Republic of Armenia
Predrag Horvat ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Božidar Šantek ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Puni tekst: engleski, pdf (385 KB) str. 113-119 preuzimanja: 659* citiraj
APA 6th Edition
Mario, N., Pavlečić, M., Harutyunyan, B., Goginyan, V., Horvat, P. i Šantek, B. (2017). Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers. Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12 (3-4), 113-119. Preuzeto s https://hrcak.srce.hr/197800
MLA 8th Edition
Mario, Novak, et al. "Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers." Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, vol. 12, br. 3-4, 2017, str. 113-119. https://hrcak.srce.hr/197800. Citirano 21.10.2020.
Chicago 17th Edition
Mario, Novak, Mladen Pavlečić, Baghish Harutyunyan, Vigen Goginyan, Predrag Horvat i Božidar Šantek. "Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers." Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam 12, br. 3-4 (2017): 113-119. https://hrcak.srce.hr/197800
Harvard
Mario, N., et al. (2017). 'Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers', Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, 12(3-4), str. 113-119. Preuzeto s: https://hrcak.srce.hr/197800 (Datum pristupa: 21.10.2020.)
Vancouver
Mario N, Pavlečić M, Harutyunyan B, Goginyan V, Horvat P, Šantek B. Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers. Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam [Internet]. 2017 [pristupljeno 21.10.2020.];12(3-4):113-119. Dostupno na: https://hrcak.srce.hr/197800
IEEE
N. Mario, M. Pavlečić, B. Harutyunyan, V. Goginyan, P. Horvat i B. Šantek, "Characteristics and selection of cultures of photosynthetic purple non-sulphur bacteria as a potential 5-aminolevulinic acid producers", Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam, vol.12, br. 3-4, str. 113-119, 2017. [Online]. Dostupno na: https://hrcak.srce.hr/197800. [Citirano: 21.10.2020.]

Sažetak
Wild strains of purple non-sulphur bacteria: Rhodospirillum rubrum B-6505, Rhodopseudomonas palustris B-6506, Rhodobacter capsulatus B-6508 and Rhodobacter spheroides B-6509 were studied as 5-ALA (5 aminolevulinic acid) producers. Selected strains were subjected to mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine to obtain a strain with high 5-ALA producing capacity. After mutagenesis 19 stable mutant strains were selected from Rhodobacter capsulatus B-6508 and Rhodobacter sphaeroides B-6509. On the basis of obtained results, mutant strain of Rhodobacter capsulatus B-6508 has shown the highest potential for 5-ALA production. The most favorable conditions for growth and 5-ALA production by mutant strain R. capsulatus B-6508 were observed in media composed of glutamate and malate, light at 2000 Lux, microaerophilic conditions and temperature of 28 °C. In these conditions, the highest 5-ALA concentration (179 mg/L) was detected together with the highest bacterial physiological activity. The prolongation of mutant strain R. capsulatus B-6508 cultivation time after glycine, succinate and levulinic acid addition is related to the reduction of 5-ALA concentrations (e.g. 124.5 mg/L after 48 h and 89.5 mg/L after 72 hours). In the light/aerobic conditions R. capsulatus B-6508 produced only 58.1 mg/L of 5 ALA. Furthermore, in dark conditions even lower biomass and 5-ALA concentrations were observed during R. capsulatus B-6508 cultivation.

Ključne riječi
purple non-sulphur bacteria; Rhodobacter capsulatus; 5-aminolevulinic acid; optimization of cultivation conditions

Hrčak ID: 197800

URI
https://hrcak.srce.hr/197800

Posjeta: 817 *