Izvorni znanstveni članak
Phenolic Compounds Composition of Hypericum perforatum L. Wild-Growing Plants from the Republic of Macedonia
Oliver Tusevski
orcid.org/ORCID
; Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Department of Plant Physiology
Marija Krstikj
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; Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Department of Plant Physiology, Archimedova str. 3, 1000 Skopje, Republic of Macedonia
Jasmina Petreska Stanoeva
orcid.org/ORCID
; Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Department of Analytical Chemistry, Archimedova str. 3, 1000 Skopje, Republic of Macedonia
Marina Stefova
orcid.org/ORCID
; Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Department of Analytical Chemistry, Archimedova str. 3, 1000 Skopje, Republic of Macedonia
Sonja Gadzovska Simic
orcid.org/ORCID
; Ss. Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Department of Plant Physiology, Archimedova str. 3, 1000 Skopje, Republic of Macedonia
Sažetak
The aim of this study was to provide comprehensive understanding of phenolic compounds composition in roots (RO), non-flower shoots (NFS) and flower shoots (FS) of Hypericum perforatum wild-growing plants from the Republic of Macedonia. Identification of phenolic compounds in plant methanolic extracts was performed by HPLC-DAD/ESI-MS analysis. Chlorogenic acid and 3-p-coumaroylquinic acid were identified in NFS and FS, while 3-feruloylquinic acid was detected in RO and FS extracts. From the group of flavan- 3-ols, (epi)catechin and procyanidins were found in all tested samples, whereas catechin and B-type procyanidin dimer were confirmed in NFS and FS. Four flavonol glycosides (hyperoside, rutin, quercitrin and kaempferol 3-O-rutinoside) were identified in aerial parts. Guaijaverin and kaempferol 3-O-glucoside were exclusively found in NFS. Quercetin, amentoflavone and I3-II8 biapigenin as flavonoid aglycones were detected only in FS extracts. The NFS and FS extracts showed a capability for the accumulation of cyanidin 3-O-glycoside and cyanidin 3-O-rhamnoside, as well for hyperforin and adhyperforin. Naphthodianthrones were represented with pseudohypericin, hypericin and protopseudohypericin in FS, while only hypericin was detected in NFS. Six xanthones, γ-mangostin, 5-O-methyl-2- deprenylrheediaxanthone B, garcinone C, 3,6-dihydroxy-1,5,7-trimethoxy-xanthone, cadensin G and cadensin C were exclusively confirmed in RO extracts. Padiaxanthone was detected in NFS, while dimethylmangiferin in FS extracts. The major finding of this study is the identification of novel xanthones in H. perforatum roots that could be potentially used as bioactive compounds in food and pharmaceutical industry.
Ključne riječi
HPLC/DAD/ESI-MSn; Hypericum perforatum L.; Phenolic compounds
Hrčak ID:
218537
URI
Datum izdavanja:
25.3.2019.
Posjeta: 1.170 *