Izvorni znanstveni članak
Xylanase Production by Aspergillus niger LPB 326 in Solid-State Fermentation Using Statistical Experimental Designs
Giselle Maria Maciel
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Luciana Porto de Souza Vandenberghe
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Charles Windson Isidoro Haminiuk
; Food Technology Department, Federal University of Technology, Paraná (UTFPR), P.O. Box 271, Campo Mourão, PR, Brazil
Ricardo Cancio Fendrich
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Bianca Eli Della Bianca
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Tahiana Quintella da Silva Brandalize
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Ashok Pandey
; Biotechnology Division, Regional Research Laboratory, CSIR, IN-695 019 Trivandrum, India
Carlos Ricardo Soccol
; Bioprocess Engineering and Biotechnology Division, Federal University of Paraná (UFPR), P.O. Box 19011, Curitiba, PR, Brazil
Sažetak
Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO4 0.4, KH2PO4 1.5 and CoSO4 0.0012, and incubated for 4 days at 30 °C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.
Ključne riječi
xylanase; Aspergillus niger; solid-state fermentation; statistical designs
Hrčak ID:
24407
URI
Datum izdavanja:
13.6.2008.
Posjeta: 5.476 *