Original scientific paper

https://doi.org/10.15255/KUI.2021.004

Validation of the ICP-MS Method for Determination of Trace Elements Provided by ICH Q3D Guideline in Fosamprenavir Calcium

Irena Radić ; Pliva Hrvatska d. o. o., TEVA API Croatia, Prudnička cesta 54, 10 000 Zagreb, Croatia
Mislav Runje ; Pliva Hrvatska d. o. o., TEVA API Croatia, Prudnička cesta 54, 10 000 Zagreb, Croatia
Matija Cvetnić orcid.org/0000-0002-0168-8092 ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia
Maja Halužan ; Pliva Hrvatska d. o. o., TEVA API Croatia, Baruna Filipovića 25, 10 000 Zagreb, Croatia
Mario-Livio Jeličić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, 10 000 Zagreb, Croatia
Martina Miloloža orcid.org/0000-0003-3719-8359 ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia
Dajana Kučić Grgić orcid.org/0000-0001-8427-6482 ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia
Viktorija Prevarić orcid.org/0000-0002-6273-7927 ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia
Šime Ukić orcid.org/0000-0001-6567-459X ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia
Tomislav Bolanča ; Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10 000 Zagreb, Croatia

Abstract

Fosamprenavir calcium is an active pharmaceutical ingredient (API) in which trace elements must be monitored by ICH Q3D Guideline. It delivers appropriate method to ensure the quality of the product and its safe use. Elemental impurities in API are separated in three classes based on their toxicity and probability of their occurrence in the product. ICP-MS has proven to be a suitable technique with the appropriate sample preparation method. Due to the presence of oxidising agents in the sample preparation, determination of osmium is problematic. In the presence of oxidising nitric acid, a highly volatile and toxic osmium tetraoxide is formed. Due to its high volatility, the recovery of osmium has reached the value of 287 %, which exceeds recovery limit (70–150 %). However, osmium can be stabilised by complexation with thiourea in the hydrochloric acid solution (recovery = 87 %). In that way, the loss of osmium is reduced and good results are achieved in terms of usability, accuracy, and precision.
A method with addition of thiourea has been successfully validated through main validation parameters: linearity, quantitation limit, selectivity, accuracy, precision (which included repeatability and intralaboratory reproducibility), and robustness. Each of these validation parameters met the acceptance criteria, and therefore it was concluded that the method is suitable for the determination of trace elements in fosamprenavir calcium by ICP-MS.




This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

microwave digestion; ICP-MS; API; fosamprenavir calcium; osmium

Hrčak ID:

264641

URI

https://hrcak.srce.hr/264641

Publication date:

2.11.2021.

Article data in other languages: croatian

Visits: 1.035 *