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Cellulase and Polygalacturonase Activity by Trichoderma viride

Anatolii Anatoliiovych Pavlenko orcid.org/0000-0001-8397-916X ; Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, Chernihiv, Ukraine
Evgeniy Pavlovych Kopilov ; Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, Chernihiv, Ukraine
Olena Volodymyrivna Nadkernychna orcid.org/0000-0002-6614-5056 ; Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, Chernihiv, Ukraine
Hanna Viktorivna Tsekhmister ; Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, Chernihiv, Ukraine
Anna Serhiivna Kyslynska ; Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS, Chernihiv, Ukraine

Sažetak

In this study the ability of Trichoderma viride IMB F-100076 to synthesize cellulase and pectinase enzymes was investigated. Clear zones in agar medium with Na-CMC, cellobiose and pectin as carbon sources were identified at different pH values. It was found that the mentioned fungus had cleaved Na-CMC, cellobiose and pectin, forming clear zones. The optimal pH value for cellulase and pectinase synthesis amounted to 5.0. At this value the clear zone and radial growth rate of T. viride was the highest. The ability of T. viride IMB F-100076 to synthesize cellulase enzymes and polygalacturonase was quantitatively proven. The maximum cellulase activity in static culture was reported on day 15 of cultivation. The total cellulase activity was 0.88 U mL-1, exoglucanase – 0.76 U mL-1, endoglucanase – 1.25 U mL-1 and β-glucosidase – 1.27 U mL-1. The highest value of polygalacturonase activity was 0.59 ± 0.01 U g-1 IDS reported at day 6 of cultivation.

Ključne riječi

Trichoderma viride, cellulase activity, endoglucanase; exoglucanase, β-glucosidase, pectinolytic enzymes, polygalacturonase

Hrčak ID:

274546

URI

https://hrcak.srce.hr/274546

Datum izdavanja:

29.3.2022.

Posjeta: 574 *