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Effect of Flavonoids on Glutathione Level, Lipid Peroxidation and Cytochrome P450 CYP1A1 Expression in Human Laryngeal Carcinoma Cell Lines

Ksenija Durgo ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Pierottijeva 6, HR-10000 Zagreb, Hrvatska
Lidija Vuković ; Institut Ruđer Bošković, Bijenička 54, HR-10000 Zagreb, Hrvatska
Gordana Rusak ; Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, Rooseveltov trg 6, HR-10000 Zagreb, Hrvatska
Maja Osmak ; Institut Ruđer Bošković, Bijenička 54, HR-10000 Zagreb, Hrvatska
Jasna Franekić Čolić ; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Pierottijeva 6, HR-10000 Zagreb, Hrvatska


Puni tekst: engleski pdf 219 Kb

str. 69-79

preuzimanja: 2.464

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Sažetak

Flavonoids are phytochemicals exhibiting a wide range of biological activities, among which are antioxidant activity, the ability to modulate activity of several enzymes or cell receptors and possibility to interfere with essential biochemical pathways. Using human laryngeal carcinoma HEp2 cells and their drug-resistant CK2 subline, we examined the effect of five flavonoids, three structurally related flavons (quercetin, fisetin, and myricetin), one flavonol (luteolin) and one glycosilated flavanone (naringin) for: (i) their ability to inhibit mitochondrial dehydrogenases as an indicator of cytotoxic effect, (ii) their influence on glutathione level, (iii) antioxidant/prooxidant effects and influence on cell membrane permeability, and (iv) effect on expression of cytochrome CYP1A1. Cytotoxic action of the investigated flavonoids after 72 hours of treatment follows this order: luteolin>quercetin>fisetin>naringin>myricetin. Our results show that CK2 were more resistant to toxic concentrations of flavonoids as compared to parental cells. Quercetin increased the total GSH level in both cell lines. CK2 cells are less perceptible to lipid peroxidation and damage caused by free radicals. Quercetin showed prooxidant effect in both cell lines, luteolin only in HEp2 cells, whereas other tested flavonoids did not cause lipid peroxidation in the tested cell lines. These data suggest that the same compound, quercetin, can act as a prooxidant, but also, it may prevent damage in cells caused by free radicals, due to the induction of GSH, by forming less harmful complex. Quercetin treatment damaged cell membranes in both cell lines. Fisetin caused higher cell membrane permeability only in HEp2 cells. However, these two compounds did not enhance the damage caused by hydrogen peroxide. Quercetin, naringin, myricetin and fisetin increased the expression of CYP1A1 in both cell lines, while luteolin decreased basal level of CYP1A1 only in HEp2 cells. In conclusion, small differences in chemical structure of flavonoids led to drastic change of their biological effects.

Ključne riječi

flavonoids; tumor cells; cytotoxicity; glutathione; lipid peroxidation; cytochrome 1A1

Hrčak ID:

30439

URI

https://hrcak.srce.hr/30439

Datum izdavanja:

15.3.2007.

Podaci na drugim jezicima: hrvatski

Posjeta: 3.488 *