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Food Technology and Biotechnology, Vol. 47 No. 1, 2009.

Izvorni znanstveni članak

Scavenging Activity of Enzymatic Hydrolysates from Wheat Bran

Jing Wang ; College of Chemistry and Environment Engineering, Beijing Technology and Business University, 11 Fucheng Road, CN-100037 Beijing, PR China
Xiaoping Yuan ; Department of Science and Technology, China Grain Reserves Corporation, 143A Xizhimenwai Street, CN-100044 Beijing, PR China
Baoguo Sun ; College of Chemistry and Environment Engineering, Beijing Technology and Business University, 11 Fucheng Road, CN-100037 Beijing, PR China
Yuan Tian ; College of Chemistry and Environment Engineering, Beijing Technology and Business University, 11 Fucheng Road, CN-100037 Beijing, PR China
Yanping Cao ; College of Chemistry and Environment Engineering, Beijing Technology and Business University, 11 Fucheng Road, CN-100037 Beijing, PR China

Puni tekst: engleski pdf 127 Kb

str. 39-46

preuzimanja: 1.157

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Sažetak

Wheat bran was destarched and deproteinated by α-amylase, protease and amyloglucosidase successively, and further hydrolyzed using Bacillus subtilis xylanases. The yield of enzymatic hydrolysates from wheat bran (EHWB) was 1.84 %. The total phenolics were 0.3712 g of ferulic acid equivalents per gram of EHWB. The antioxidant potency of EHWB was evaluated using different assays, such as iron ion chelation, reducing power, scavenging activity against 2,2’-diphenyl-1-picrylhydrazyl (DPPH) and reactive oxygen species under in vitro conditions. EHWB exhibited an effective ferrous ion chelating activity and strong reducing power. It also showed a high DPPH radical scavenging activity (89.4 %) at 5.0 mg/mL, which was comparable to that of the synthetic antioxidant, butylated hydroxytoluene. EHWB also exerted a marked scavenging effect on ·OH with an EC50 value of 0.46 mg/mL, which was lower than that of mannitol (1.03 mg/mL), a classical hydroxyl radical scavenger, and obvious antioxidant activities toward O2·- and H2O2.

Ključne riječi

antioxidant activity; enzymatic hydrolysates; iron chelation; oxidative stress; wheat bran; xylanases

Hrčak ID:

33051

URI

https://hrcak.srce.hr/33051

Datum izdavanja:

26.3.2009.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.963 *