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Clenbuterol Residues in Plasma and Urine Samples of Food-Producing Pigs During and After Subchronic Exposure to a Growth-Promoting Dose
Jelka Pleadin
orcid.org/0000-0002-0768-0462
; Laboratorij za analitičku kemiju, Hrvatski veterinarski institut, Savska 143, HR-10 000 Zagreb, Hrvatska
Tihomira Gojmerac
; Laboratorij za analitičku kemiju, Hrvatski veterinarski institut, Savska 143, HR-10 000 Zagreb, Hrvatska
Igor Bratoš
; Pliva Istraživački institut, Prilaz Baruna Filipovića 29, HR-10 000 Zagreb, Hrvatska
Zoran Lipej
; Laboratorij za opću patologiju, Hrvatski veterinarski institut, Savska 143, HR-10 000 Zagreb, Hrvatska
Dinko Novosel
; Laboratorij za opću patologiju, Hrvatski veterinarski institut, Savska 143, HR-10 000 Zagreb, Hrvatska
Ana Vulić
; Laboratorij za analitičku kemiju, Hrvatski veterinarski institut, Savska 143, HR-10 000 Zagreb, Hrvatska
Sažetak
The aim of the study is to evaluate the suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmation method. A high correlation coefficient between these analytical methods was obtained for both urine (R=0.9800) and plasma (R=0.9970) concentrations. Study results show the plasma and urine concentration to vary greatly during oral treatment with clenbuterol for 28 days. The peak urine concentration ((88.54±50.54) ng/mL) recorded on day 21 was 40-fold peak plasma concentration ((2.25±1.54) ng/mL). After withdrawal period, the peak urine clenbuterol concentration ((42.93±10.52) ng/mL) recorded on day 0 was 24-fold plasma concentration ((1.79±0.97) ng/mL). The maximum allowed concentration of 0.5 ng/g in the liver as a regulated matrix for control of clenbuterol abuse was achieved in plasma on day 3 ((0.52±0.26) ng/mL) and in urine on day 7 of treatment withdrawal ((0.45±0.11) ng/mL). Study results indicate that urine and plasma may be suitable matrices for the control of clenbuterol abuse during fattening of food-producing pigs but have a limited value because of the rapidly decreasing concentration upon treatment withdrawal, in plasma in particular.
Ključne riječi
clenbuterol residues; growth promoting dose; subchronic exposure; pig; urine; plasma
Hrčak ID:
33055
URI
Datum izdavanja:
26.3.2009.
Posjeta: 2.145 *