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Hrana u zdravlju i bolesti : znanstveno-stručni časopis za nutricionizam i dijetetiku, Vol. Specijalno izdanje No. Apiterapija za djecu, 2024.

Izvorni znanstveni članak

THE WATER-SOLUBLE PROPOLIS SHOWS ANTI – INFLUENZA VIRUS ACTIVITY

Bratko Filipič ; Croatian Institute for Experimental and Translational Oncology – CIETO, Koledinećka 3, 10040 Zagreb, Croatia *
Klemen Rihar ; Retired, Chengdujska, Ljubljana, Slovenia
Dunja Exel Gregorič ; Retired, Glavarjeva, Ljubljana, Slovenia
Adriana Pereyra ; Medex d.o.o., Linhartova cesta 49a, 1000 Ljubljana, Slovenia
Hrvoje Mazija ; Croatian Institute for Experimental and Translational Oncology – CIETO, Koledinećka 3, 10040 Zagreb, Croatia

* Dopisni autor.

Puni tekst: engleski pdf 454 Kb

str. 1-8

preuzimanja: 411

citiraj

Sažetak

The Influenza virus affects the respiratory tract in humans, causing a range of distinct manifestations including fever, nasal secretions, cough, headaches, muscle pain and pneumonia, which could become violent and severe. Influenza A viruses remain resistant to Amantadine and Rimantadine with a high level of Oseltamivir. Therefore, there is a need for constant improvement of drugs active against resistant Influenza viruses. Propolis has anti-influenza activity both in vitro and in vivo. Human leukocyte interferon (HuIFN-αN3) is a multi-subtype protein that displays activity against Influenza A, B, and C viruses.
In this study, authors elucidated the anti - Influenza activity of the mixes of Water extract of Propolis and HuIFN-αN3 at different ratios:1:1,1:2 and 2:1. Water extract of Propolis's polyphenols and HuIFN-αN3 were characterized by RP-HPLC. Influenza A and B viruses were separately added to the LLC-MK2 cells treated with Water extract of Propolis and HuIFN-αN3 alone or in ratios 1:1, 1:2, and 2:1.Plates were incubated and cytopathic effects were determined. The best results of ID50 were obtained with the mix of 10% Water extract of Propolis and HuIFN-αN3 1:2, showing ID50 at 12 ± 0.2 μg/mL for Influenza A and 19 ± 0.6 μg/mL for Influenza B viruses. When comparing the anti-influenza activity of the Water extract of Propolis /HuIFN-αN3 with that of Ribavirin, it was found that 1:2 was the optimal ratio for Water extract of Propolis /HuIFN-αN3 (0.5 and 0.6 for Influenza A and B). This new formulation of Water extract of Propolis and HuIFN-αN3, showing better anti-influenza activity, will improve its application in children' s flu infections in vivo.

Ključne riječi

Ethanol extract of Propolis; Influenza viruses; Antiviral activity; Tissue culture

Hrčak ID:

330926

URI

https://hrcak.srce.hr/330926

Datum izdavanja:

31.12.2024.

Posjeta: 575 *