Skoči na glavni sadržaj

Hrvatski športskomedicinski vjesnik, Vol. 18 No. 1-2, 2003.

Izvorni znanstveni članak

Evaluation of blood testing methodology for use in sport

Harm Kuipers ; Dept. of movement sciences, University of Maastricht; International Skating Union
Tim Brouwer ; Dept. of movement sciences, University of Maastricht
Steven Vanhoutvin ; Dept. of movement sciences, University of Maastricht
Sanda Dubravcic-Simunjak ; International Skating Union
Jane Moran ; International Skating Union
David Mitchel ; International Skating Union
Joel Shobe ; International Skating Union
Hiroya Sakai ; International Skating Union
James Stray-Gundersen ; International Skating Union

Puni tekst: engleski pdf 101 Kb

str. 10-14

preuzimanja: 117

citiraj

Sažetak

Aim: to study the effect of posture, duration of sto- at room temperature (20oC) or in the
refrigerator rage (8oC), time of the day, as well as intra-sample variability and possible differences between two hematological ana- lyzers, on hematological variables.
Methods: in 13 healthy subjects we conducted a series of experiments. For inter-analyzer comparison a number of samples was analyzed both on an Advia-120 and on a Gen-S analyzer.
Results: comparing standing and 5 minutes sitting, no difference in hemoglobin (Hb) and hematocrit (Ht) was found. However, the number of reticulocytes was lower (p<0.01) after 5 minutes sitting (44.1±6.5 x 109/l) com- pared to standing (69.1±4.8 x 109/l). Storage at room 20oC or 8oC for 8 hours did not affect hemoglobin or hematocrit, although the mean number of reticulocytes increased from 58.3±22.3 x 109/l to 74.2±21.0 x 109/l after 8 hours in both cases. Hematological variables did not change signi- ficantly during the day, except for the number of reticu- locytes that peaked at 5 pm (78.1±7.3 x 109/l) compared to 8 am (57.5±7.1 x 109/l). On the Advia 120 (Bayer) hemoglobin, hematocrit, and number of reticulocytes were slightly but consistently higher (p<0.001) compared to the General-S (Coulter).
Conclusion: before blood draw in sport, the athletes should be seated for 5 minutes. For reticulocyte count the samples should preferably be analyzed within 8 hours at unique analizator.

Ključne riječi

sport, blood testing, hematological variables

Hrčak ID:

330993

URI

https://hrcak.srce.hr/330993

Datum izdavanja:

30.12.2003.

Posjeta: 262 *