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Journal of Electrochemical Science and Engineering, Vol. 15 No. 5, 2025.

Original scientific paper

https://doi.org/10.5599/jese.2705

Covalent attachment of aminoferrocene to pseudo-graphite for selective sensing of H2O2

Forrest Dalbec ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA
Jeremy May ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA
Dipak Koirala ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA
Jhonnathan A. Plascencia ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA
Micheal Okeke ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA
Joshua Russell ; Micron School of Materials Science and Engineering Boise State University Boise ID 83725, USA
Hui Xiong ; Micron School of Materials Science and Engineering Boise State University Boise ID 83725, USA
I. Francis Cheng ; Department of Chemistry, University of Idaho, 875 Perimeter Dr, Moscow, ID-83844, USA *

* Corresponding author.

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Abstract

Hydrogen peroxide is an important analyte from both the standpoints of in vivo and in vitro analyses. Electrochemical methods offer good prospects, but present sensing methods suffer from inadequate limits of detection (LOD), low sensitivity, poor stability, and nume­rous interferents. To address these issues, we use the recently recognized peroxidase-like activity of ferrocene for sensing based on the reductive electrocatalysis of H2O2. This route obviates interferences from ascorbate, urea, urate, dopamine and glucose. Immo­bilization of ferrocene overcomes its low aqueous solubility. This is done by the chemical modification of a pseudo-graphite material rich in sp3 defects. The surface is modified for carboxylate features through a diazonium intermediate formed through 4-aminobenzoic acid. Amino­ferrocene is attached to surface carboxylate groups through a N-ethyl-N’-(3-(di­me­thyl­ami­no)propyl) carbodiimide coupling agent. Cyclic voltametric studies indicate that the surface concentration of ferrocene is 1.06 nmol cm-2. Chronoamperometric response at -0.6 V vs. Ag/AgCl, gives a LOD of 0.070 µM with a high sensitivity of 553.2 μA mM-1 cm-2. This is the best sensitivity reported in the literature. Furthermore, the sensor is stable over 50 cyclic voltametric scans, and between the 3 calibration-curve studies.

Keywords

Peroxide detection; ferrocene; interference-free; diazonium; graphitic carbon

Hrčak ID:

335079

URI

https://hrcak.srce.hr/335079

Publication date:

9.7.2025.

Visits: 149 *