Izvorni znanstveni članak
https://doi.org/10.15644/asc59/4/5
Cross-Sectional Analysis of Matrix Metalloproteinases and Bone Resorption Regulators in Apical Periodontitis: Linking Molecular Mechanisms with Clinical and Histopathological Features
Dobroslav Purešević
; Istraživački laboratoriji Implantološko-istraživačkoga centra, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Ema Krdžovic Lazić
; Istraživački laboratoriji Implantološko-istraživačkoga centra, Stomatološki fakultet, Sveučilište u Beogradu, Srbija; Klinika za bolesti zuba, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Nađa Nikolić
; Istraživački laboratoriji Implantološko-istraživačkoga centra, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Ognjan Pavlović
; Istraživački laboratoriji Implantološko-istraživačkoga centra, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Francesca Ideo
; Klinika za konzervativnu stomatologiju i endodonciju, Sveučilište u Cagliariju, Italija
Miroslav Andrić
; Klinika za oralnu hirurgiju, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Maja Miletić
; Odsjek za patološku fiziologiju, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
Aleksandar Jakovljević
orcid.org/0000-0001-6512-4934
; Istraživački laboratoriji Implantološko-istraživačkoga centra, Stomatološki fakultet, Sveučilište u Beogradu, Srbija;
*
Katarina Beljić-Ivanović
; Klinika za bolesti zuba, Stomatološki fakultet, Sveučilište u Beogradu, Srbija
* Dopisni autor.
Sažetak
Objective(s) : (a) To quantify the relative gene expression of matrix metalloproteinases (MMP) -2, -9, their tissue inhibitor (TIMP) -2, receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in human apical periodontitis (AP) and to associate it with levels of these molecules in healthy dental pulps. (b) To assess differences in gene expression among AP lesions stratified by clinical, radiographic, and histopathological features. (c) Finally, a potential correlation of investigated molecules was explored in AP samples. Materials and Methods: The study cohort comprised 80 AP lesions obtained from 80 adult volunteers during apicoectomy procedures. The healthy control cohort consisted of 50 dental pulp samples from intact teeth extracted for orthodontic purposes, collected from 50 voluntary donors. Relative gene expression of MMP-2, MMP-9, TIMP-2, RANKL, and OPG was assessed in all tissue samples using reverse transcription quantitative realtime polymerase chain reaction (RT-qPCR). Statistical analyses included the Chi-square test, Student’s t-test, Mann–Whitney U test, and Spearman correlation. Results: Higher gene expression levels of RANKL, OPG, MMP-2, and MMP-9 were observed in the study compared to the healthy cohort (p=0.048, p=0.002, p=0.048, and p=0.012, respectively). Higher relative expression of the MMP2 gene was observed in smaller lesions compared to large lesions (p=0.021). Significant positive correlations were observed between the investigated mediators. Conclusions: Concurrent overexpression of the studied mediators, along with their positive correlations in AP lesions, indicates their coordinated role in disease progression and alveolar bone resorption.
Ključne riječi
Periapical Periodontitis; Inflammation Mediators; Matrix Metalloproteinases; Bone Resorption; Alveolar Bone Loss; Apical Periodontitis; Inflammatory mediators, Matrix Metalloproteinases; Bone Resorption
Hrčak ID:
341916
URI
Datum izdavanja:
26.12.2025.
Posjeta: 674 *