Izvorni znanstveni članak

https://doi.org/10.20471/acc.2025.64.03.13

Expression Profile of ADIPOQ and TCF7L2 Genes in Type 2 Diabetic Pakistani Subjects

Saima Sharif ; Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Saira Rafaqat orcid.org/0000-0001-7177-5602 ; Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Abeerah Saleem ; Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Saima Nasir ; Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Shagufta Naz ; Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Aleksandra Klisić orcid.org/0000-0001-7870-0996 ; University of Montenegro, Faculty of Medicine, Podgorica, Montenegro; Center for Laboratory Diagnostics, Primary Health Care Center, Podgorica, Montenegro *

* Dopisni autor.

Sažetak

The purpose of this study was to investigate the expression of the ADIPOQ and
transcription factor 7-like 2 (TCF7L2) genes in type 2 diabetes mellitus (T2D) in a Pakistani population.
This aim of the study was to find the potential link between the expression of the ADIPOQ and TCF7L2
genes in T2D. This cross-sectional study included 200 patients with T2D and 200 control subjects.
Analysis of the expression of the ADIPOQ and TCF7L2 genes was performed by real-time polymerase
chain reaction. A significant increase in the expression of ADIPOQ and TCF7L2 genes was observed in
T2D group as compared to control group. In T2D group, a significant positive relationship was found
between the expression of the ADIPOQ gene and systolic blood pressure, random blood glucose and
expression of TCF7L2 gene. A significant negative correlation was observed between the expression of
the TCF7L2 gene and diastolic blood pressure. Multivariable linear regression analysis revealed that the
expression of TCF7L2 gene (β=0.274; adjusted R2 change, 0.135; p=0.005) and expression of ADIPOQ
gene (β=0.270; adjusted R2 change, 0.148; p=0.001) were positively associated in T2D, whereas diastolic
blood pressure (β=-0.296; adjusted R2 change, 0.148; p=0.001) was negatively associated in T2D as
compared to control group. When stepwise linear regression was performed considering the expression
of the TCF7L2 gene as a dependent variable in T2D group, one model was computed showing the
expression of ADIPOQ gene (β=0.335, p=0.001) as an important predictor of T2D. When stepwise linear
regression was employed considering the expression of ADIPOQ gene as a dependent variable, two
models were computed showing the expression of TCF7L2 gene (β=0.335; p=0.001) and systolic blood
pressure (β=0.138; p=0.041) as important determinators of T2D. Thus, the expression of the ADIPOQ
and TCF7L2 genes are independent predictors of T2D. In conclusion, there was increased expression
of the ADIPOQ gene and TCF7L2 gene in T2D subjects as compared to controls. The ADIPOQ gene
expression and TCF7L2 gene expression provide independent predictors of T2D indicating their role
in the development of T2D. Future research is required to elucidate the mechanisms involved in the
increased ADIPOQ and TCF7L2 gene expression in T2D.

Ključne riječi

Type 2 diabetes; ADIPOQ gene; TCF7L2 gene; Gene expression; Pakistani population

Hrčak ID:

342939

URI

https://hrcak.srce.hr/342939

Datum izdavanja:

30.9.2025.

Podaci na drugim jezicima: hrvatski

Posjeta: 365 *