Izvorni znanstveni članak

https://doi.org/10.20471/acc.2025.64.03.14

Does Lipemia Affect Common Coagulation Tests?

Tajana Tunjić ; Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Croatia; University of Applied Health Sciences, Zagreb, Croatia
Dragana Šegulja orcid.org/0000-0002-6894-2358 ; Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Croatia; University of Applied Health Sciences, Zagreb, Croatia *
Dunja Rogić ; Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Croatia; Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia

* Dopisni autor.

Sažetak

Lipemia is a common preanalytical source of errors and sample rejection, especially
in photometric assays. The aim of this study was to assess the effect of lipemia on the most common
coagulation tests; prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen
and antithrombin.
Leftover citrate plasma samples were used to prepare the plasma pool which was subsequently spiked
with raising amounts of lipid solutions. All tests were performed on a BCSXP® analyser (Siemens, Erlangen,
Germany) utilizing the reagents from the same manufacturer. The effect of lipemia was assessed
by comparing the difference in results between the native sample, the sample with the added interferent
and the reference change value (RCV). Additionally, the correctness of the reaction curve of each analyte
was taken into account.
PT results were not affected by triglyceride concentrations below 26.1 mmol/L when measured at
570 nm. However, if measured at 405 nm, results were acceptable only when triglyceride concentrations
were not higher than 14.4 mmol/L. For APTT results, triglyceride concentrations below 12.8 mmol/L
at 405 nm and below 20.3 mmol/L for 570 nm met eligibility criteria. Fibrinogen values are acceptably
accurate only when triglyceride concentrations were under 12.8 mmol/L. Finally, no clinically significant
triglyceride interference was found for the antithrombin test results with triglyceride concentrations of
up to 37.8 mmol/L.
Interfering substances may impact coagulation assay results differently, depending on the parameter
and method reaction. In this study, the threshold of lipid interferences was established for PT, APTT
and fibrinogen, as well as for antithrombin measurements, which proved to be the least affected.

Ključne riječi

preanalytical phase; lipemia; coagulation tests; interference; urgent laboratory

Hrčak ID:

342940

URI

https://hrcak.srce.hr/342940

Datum izdavanja:

30.9.2025.

Podaci na drugim jezicima: hrvatski

Posjeta: 456 *