In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic

Adelusi, Oludotun O.; Oderinwale, Olatunde A.; Alabi, Joel O.; Wuaku, Michael; Enikuomehin, James M.; Anotaenwere, Chika C.; Okedoyin, Deborah O.; Ike, Kelechi A.; Kholif, Ahmed E.; Subedi, Kiran; Anele, Uchenna Y.

doi:10.5513/WOSL5646

Agriculturae Conspectus Scientificus, Vol. 91 No. 1, 2026.

Izvorni znanstveni članak

In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic

Oludotun O. Adelusi ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Olatunde A. Oderinwale ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Joel O. Alabi orcid.org/0000-0003-1442-5213 ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Michael Wuaku ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
James M. Enikuomehin ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Chika C. Anotaenwere ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Deborah O. Okedoyin ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Kelechi A. Ike ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Ahmed E. Kholif ; Department of Animal Sciences, North Carolina Agricultural and Technical State University
Kiran Subedi ; Analytical Services Laboratory, College of Agriculture and Environmental Sciences, North Carolina Agricultural and Technical State University
Uchenna Y. Anele orcid.org/0000-0002-6630-9292 ; Department of Animal Sciences, North Carolina Agricultural and Technical State University *

* Dopisni autor.

Puni tekst: engleski pdf 583 Kb

preuzimanja: 28

citiraj

APA 6th Edition

Adelusi, O.O., Oderinwale, O.A., Alabi, J.O., Wuaku, M., Enikuomehin, J.M., Anotaenwere, C.C., ... Anele, U.Y. (2026). In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic. Agriculturae Conspectus Scientificus, 91 (1). https://doi.org/10.5513/WOSL5646

MLA 8th Edition

Adelusi, Oludotun O., et al. "In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic." Agriculturae Conspectus Scientificus, vol. 91, br. 1, 2026 https://doi.org/10.5513/WOSL5646. Citirano 25.05.2026.

Chicago 17th Edition

Adelusi, Oludotun O., Olatunde A. Oderinwale, Joel O. Alabi, Michael Wuaku, James M. Enikuomehin, Chika C. Anotaenwere, Deborah O. Okedoyin, et al. "In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic." Agriculturae Conspectus Scientificus 91, br. 1 https://doi.org/10.5513/WOSL5646

Harvard

Adelusi, O.O., et al. (2026). 'In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic', Agriculturae Conspectus Scientificus, 91(1). https://doi.org/10.5513/WOSL5646

Vancouver

Adelusi OO, Oderinwale OA, Alabi JO, Wuaku M, Enikuomehin JM, Anotaenwere CC i sur. In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic. Agriculturae Conspectus Scientificus [Internet]. 2026 [pristupljeno 25.05.2026.];91(1). https://doi.org/10.5513/WOSL5646

IEEE

O.O. Adelusi, et al., "In vitro fermentation characteristics of calf grower diet incubated with essential oil blend and Lactobacillus-derived probiotic and postbiotic", Agriculturae Conspectus Scientificus, vol.91, br. 1, 2026. [Online]. https://doi.org/10.5513/WOSL5646

Puni tekst: hrvatski pdf 583 Kb

preuzimanja: 32

citiraj

APA 6th Edition

MLA 8th Edition

Chicago 17th Edition

Harvard

Vancouver

IEEE

Sažetak

This study evaluated gas production and nutrient digestibility of calf grower feed containing Lactobacillus plantarum (LP) incubated with postbiotic Lactobacillus acidophilus (PLA) and an essential oil blend (EOB). Eight treatments were
arranged in a 2 × 4 factorial arrangement with two feeds (with or without LP) and four additives (no additive, PLA, EOB and PLA+EOB), with eight replicates per treatment (4 bottles per run across two independent incubation runs). Treatments were randomly assigned to incubation bottles within each run, and each bottle was considered an experimental unit. PLA and EOB were administered at 5 and 20 μL/g of substrate, respectively. An in-vitro batch culture was conducted for 24 h. Data were analyzed using two-way ANOVA. Results showed that EOB
increased microbial mass, while the combination of LP, PLA and EOB improved the partitioning factor. LP and PLA increased total gas, whereas EOB alone or with PLA reduced methane production and increased hydrogen sulfide. Feed digestibility was largely unaffected, although EOB increased neutral detergent fiber digestibility and reduced total volatile fatty acid concentration. It is concluded that probiotic L. plantarum and postbiotic L. acidophilus supplementation enhanced total gas production, while EOB reduced methane emissions, and additive combinations improved microbial and fermentative efficiency.