Original scientific paper

MATURE EMBRYO AS A SOURCE MATERIAL FOR EFFICIENT REGENERATION RESPONSE IN SORGHUM (Sorghum bicolor L. Moench.)

Sudhakar POLA ; Andhra University, Visakhapatnam, India
N. SARADAMANI ; Andhra University, Visakhapatnam, India
T. RAMANA ; Andhra University, Visakhapatnam, India

Abstract

Efficient plant regeneration is a prerequisite for a complete genetic
transformation protocol in cereals. Aiming this, in the present study, we have
accomplished efficient plant regeneration using mature embryos as a source
material in Sorghum bicolor (L.) Moench. Although immature inflorescence
and immature embryos are best explant sources for in vitro culture in
Sorghum, however they are available only for a limited period in a year.
Mature embryos have always been ideal for in vitro studies for the reason
that they can be handled easily over other explants and available throughout
the year.
Mature embryo explants of Sorghum bicolor genotypes viz. IS 3566, SPV
475, CSV13, CSV15, CSV112, IS 348 were cultured on MS medium for
efficient callus induction and subsequent plant regeneration. The response of
different combination and concentrations of plant growth regulators were
compared, and factors affecting the mature embryo tissue culture response
were studied in this manuscript. Significant genotypic differentiation was
detected in embryogenic callus induction and plantlet regeneration. Genotype
IS 3566 showed better tissue culture response than the other genotypes.
Efficient embryogenic callus induction was achieved with 2mg l-1 2, 4,5-
Trichlorophenoxyacetic acid (2,4,5-T) and multiple shoot induction was
achieved by manipulation of 6-benzyl adenine (BAP), Thidiazuron (TDZ),
and Indole-3-acetic acid (IAA) in the culture medium.

Keywords

Sorghum bicolor; embryos; 2; 4; 5-T; embryogenic callus; BAP; TDZ; regeneration

Hrčak ID:

48648

URI

https://hrcak.srce.hr/48648

Publication date:

15.1.2010.

Article data in other languages: croatian

Visits: 2.034 *