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Concentration-dependent Effects of Peroxovanadium Compound bpV(phen) on PC12 Cell Survival

Lada Rumora
Karmela Barišić
József Petrik
Tihana Žanić Grubišić


Puni tekst: engleski pdf 155 Kb

str. 419-425

preuzimanja: 811

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Sažetak

Peroxovanadium compounds are potent insulinomimetic agents and protein tyrosine phosphatase inhibitors. In this study, the potential toxicity of peroxovanadium complex bpV(phen) on rat pheochromocytoma PC12 cells was examined, and the mechanism by which this compound influences cell survival and/or death was explored. BpV(phen) exerted a bimodal effect on PC12 cells: survival enhancing effect at lower and death-inducing effect at higher micromolar concentrations. 1 and 3 μmol dm–3 bpV(phen) intensely induced ERK activation. In contrast, 10 and 100 μmol dm–3 bp (phen) stimulated strong and sustained JNK and p38 MAPK activation as well as caspase-3 activation that preceded bpV(phen)-induced apoptotic cell death. It is suggested that bpV(phen) might exert its action on PC12 cell survival by modulation of MAPKs and caspase-3 activation.

Ključne riječi

peroxovanadium compounds; MAPK; caspase; apoptosis; PC12 cells

Hrčak ID:

52

URI

https://hrcak.srce.hr/52

Datum izdavanja:

15.9.2005.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.043 *