Izvorni znanstveni članak
Mould Growth and Aflatoxin Accumulation Affected by Newly Synthesized Derivative of Coumarine Treatment of Maize Hybrid
Lejla Duraković
; University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottieva 6, 10000 Zagreb, Croatia
Mihaela Blažinkov
; University of Zagreb, Faculty of Agriculture, Department of Microbiology, Svetošimunska 25, 10000 Zagreb, Croatia
Branka Šeol
; University of Zagreb, Veterinary Faculty, Department for Microbiology and Infectious Disease with Clinic, Heinzelova 55, 10000 Zagreb, Croatia
Alma Tudić
; Croatian Waters, Sector of Development and Water Economy, Vukovarska 220, 10000 Zagreb, Croatia
Frane Delaš
; University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottieva 6, 10000 Zagreb, Croatia
Marijan Bošnjak
; University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Pierottieva 6, 10000 Zagreb, Croatia
Sanja Sikora
; University of Zagreb, Faculty of Agriculture, Department of Microbiology, Svetošimunska 25, 10000 Zagreb, Croatia
Andrea Skelin
; University of Zagreb, Faculty of Agriculture, Department of Microbiology, Svetošimunska 25, 10000 Zagreb, Croatia
Katarina Huić-Babić
; University of Zagreb, Faculty of Agriculture, Department of Microbiology, Svetošimunska 25, 10000 Zagreb, Croatia
Zijad Duraković
; Institute for Anthropology, Department for Medical Anthropology and Epidemiology, Gajeva 32, 10000 Zagreb, Croatia
Sažetak
The effect of newly synthesized derivative of 11-hydroxy-7-imino-7H-7a, 12-diazabenzo/α/anthracen-6-one, on growth and aflatoxin B1 and G1 (AFB1 and AFG1) accumulation by toxigenic mould Aspergillus fl avus ATCC 26949 was studied on a solid substrate (maize grains) to determine the possible use of this compound as a mean of controlling aflatoxin accumulation. Experiments were carried out in a stationary culture at temperature of 28oC during 21 days. The growth of mould was monitored by measuring the analysis of chitin as glucosamine, as a criterion, and concentration of AFB1 and AFG1 was measured by HPLC method using Hewlett-Packard instrument with fluorescence detector. Concentration of investigated coumarine of 0.05 mmol x g-1 stimulated mould growth and aflatoxin accumulation, but concentration of 0.2 mmol x g-1 or higher produced an inhibitory effect. In the presence of 0.2 and 0.5 mmol x g-1 of this compound, mould growth was decreased by 22% and 65%, respectively. Concentration of AFB1 in these investigations was reduced by 30% and 90%, and concentration of AFG1 was reduced almost completely in respect to values obtained in control experiments. In experiments with 2.0 mmol x g-1 of this inhibitor no synthesis of both investigated aflatoxins was found in the sample although small increment of biomass was detected.
Ključne riječi
moulds; chitin; aflatoxins; antifungal agents; coumarine
Hrčak ID:
66002
URI
Datum izdavanja:
20.12.2010.
Posjeta: 928 *