Veterinarski arhiv, Vol. 83 No. 1, 2013.
Izvorni znanstveni članak
The influence of organic selenium feed supplement and fasting on oxidative damage in different tissues of broiler chickens.
Jasna Aladrović
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Blanka Beer Ljubić
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Suzana Milinković Tur
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sandra Plužarić
; Student of Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The influence was investigated of organic selenium feed supplements on lipid peroxidation and protein oxidation in different tissues of Ross 308 broiler chickens of both sexes in response to 48 hours food deprivation. Chickens were randomly allocated into a standard diet fed control group (N = 50) and an experimental group, pair-fed with the same diet supplemented with 0.3 ppm organic selenium (Sel-Plex®, Alltech, Inc., KY; N = 50). At the age of 42 days and after 48 hours food deprivation, ten chickens from the experimental group and ten chickens from the control were sacrificed and the liver, kidneys and small intestines were removed. Liver, small intestine and kidney tissue homogenates were analysed for lipid peroxide concentrations (LPO) and protein carbonyl content (PCC). The PCC in kidney and small intestine of experimental chickens at the end of the fattening period was higher than in the control broilers (P<0.05). As a result of the food deprivation, lower PCC was found in the liver of both groups (P<0.05). Simultaneously, the values for the experimental group were lower than in the control group (P<0.05). Kidney PCC was only increased after fasting in the control birds (P<0.05). The LPO in the kidneys at the end of fattening was higher in chickens fed diet supplemented with organic selenium (P<0.05). Kidney and small intestine tissue LPO of both groups was decreased after fasting (P<0.05). At the same time the LPO in the liver of the experimental group was increased (P<0.05). The results of this study demonstrate that dietary organic selenium supplements increase oxidative damage in the kidneys and small intestines at the end of fattening. Food deprivation resulted in decreased tissue oxidative damage due to the lower metabolic rate.
Ključne riječi
chicken; organic selenium; food deprivation; lipid peroxidation; protein oxidation
Hrčak ID:
97122
URI
Datum izdavanja:
11.2.2013.
Posjeta: 1.300 *