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Control of chloramphenicol in samples of meat, meat products and fish

Nina Bilandžić ; Laboratorij za određivanje rezidua, Odjel za veterinarsko javno zdravstvo, Hrvatski veterinarski institut, Zagreb
Ivana Varenina ; Laboratorij za određivanje rezidua, Odjel za veterinarsko javno zdravstvo, Hrvatski veterinarski institut, Zagreb
Božica Solomun Kolanović ; Laboratorij za određivanje rezidua, Odjel za veterinarsko javno zdravstvo, Hrvatski veterinarski institut, Zagreb


Puni tekst: hrvatski pdf 161 Kb

str. 161-166

preuzimanja: 868

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Puni tekst: engleski pdf 134 Kb

str. 192-196

preuzimanja: 2.117

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Sažetak


In the goal of controlling the illegal use and the control of chloramphenicol remains, during the year 2010 there were collected samples of muscle tissue of poultry (n=33), bovine (n=109) and pigs (n=46), meat products (n=21) and fish (n=7), from all areas of the
Republic of Croatia. Concentrations of chloramphenicol were determined by the screening ELISA method and confirmatory method
of liquid chromatography- tandem mass spectrometry (LC-MS/MS). Validation of methods proved their quantification capability below
minimum required performance limit of method performance of 0.3 μg/ kg. The determined concentrations of chloramphenicol
in bovine, pig and poultry meat, meat products and fish were in the range of minimum value of 0.177 to the highest value of 202.9 ng/
kg. The lowest mean concentration of 3.54 ng/ kg was determined in meat products and the highest of 15.8 ng / kg in bovine meat.
There were no determined concentrations over 300 ng/ kg in any sample of the controlled meat and fish. Considering the determined
low concentrations of chloramphenicol, it can be concluded that the meat of controlled animals is safe to consume. That means that
the prohibition on use of that antibiotic is respected in the Republic of Croatia. In accordance with the determined cases of increased
concentrations in meat, fish and shrimps originating from Asia, a constant control of imported shipments from the countries of that
part of the world is necessary.

Ključne riječi

chloramphenicol; meat; fish; ELISA; LC-MS/MS

Hrčak ID:

72579

URI

https://hrcak.srce.hr/72579

Datum izdavanja:

15.6.2011.

Podaci na drugim jezicima: hrvatski

Posjeta: 3.665 *